1d2s
From Proteopedia
(New page: 200px<br /> <applet load="1d2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2s, resolution 1.55Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1d2s.gif|left|200px]]<br /> | + | [[Image:1d2s.gif|left|200px]]<br /><applet load="1d2s" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1d2s" size=" | + | |
caption="1d2s, resolution 1.55Å" /> | caption="1d2s, resolution 1.55Å" /> | ||
'''CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE'''<br /> | '''CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Human sex hormone-binding globulin (SHBG) transports sex steroids in blood | + | Human sex hormone-binding globulin (SHBG) transports sex steroids in blood and regulates their access to target tissues. In biological fluids, SHBG exists as a homodimer and each monomer comprises two laminin G-like domains (G domains). The crystal structure of the N-terminal G domain of SHBG in complex with 5alpha-dihydrotestosterone at 1.55 A resolution reveals both the architecture of the steroid-binding site and the quaternary structure of the dimer. We also show that G domains have jellyroll topology and are structurally related to pentraxin. In each SHBG monomer, the steroid intercalates into a hydrophobic pocket within the beta-sheet sandwich. The steroid and a 20 A distant calcium ion are not located at the dimer interface. Instead, two separate steroid-binding pockets and calcium-binding sites exist per dimer. The structure displays intriguing disorder for loop segment Pro130-Arg135. In all other jellyroll proteins, this loop is well ordered. If modelled accordingly, it covers the steroid-binding site and could thereby regulate access of ligands to the binding pocket. |
==About this Structure== | ==About this Structure== | ||
| - | 1D2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA and DHT as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2S with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb92_1.html Anabolic Steroids]]. Full crystallographic information is available from [http:// | + | 1D2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=DHT:'>DHT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1D2S with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb92_1.html Anabolic Steroids]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Avvakumov, G | + | [[Category: Avvakumov, G V.]] |
[[Category: Dales, D.]] | [[Category: Dales, D.]] | ||
[[Category: Grishkovskaya, I.]] | [[Category: Grishkovskaya, I.]] | ||
| - | [[Category: Hammond, G | + | [[Category: Hammond, G L.]] |
| - | [[Category: Muller, Y | + | [[Category: Muller, Y A.]] |
[[Category: Sklenar, G.]] | [[Category: Sklenar, G.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: steroid transport]] | [[Category: steroid transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:21 2008'' |
Revision as of 10:12, 21 February 2008
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CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE
Overview
Human sex hormone-binding globulin (SHBG) transports sex steroids in blood and regulates their access to target tissues. In biological fluids, SHBG exists as a homodimer and each monomer comprises two laminin G-like domains (G domains). The crystal structure of the N-terminal G domain of SHBG in complex with 5alpha-dihydrotestosterone at 1.55 A resolution reveals both the architecture of the steroid-binding site and the quaternary structure of the dimer. We also show that G domains have jellyroll topology and are structurally related to pentraxin. In each SHBG monomer, the steroid intercalates into a hydrophobic pocket within the beta-sheet sandwich. The steroid and a 20 A distant calcium ion are not located at the dimer interface. Instead, two separate steroid-binding pockets and calcium-binding sites exist per dimer. The structure displays intriguing disorder for loop segment Pro130-Arg135. In all other jellyroll proteins, this loop is well ordered. If modelled accordingly, it covers the steroid-binding site and could thereby regulate access of ligands to the binding pocket.
About this Structure
1D2S is a Single protein structure of sequence from Homo sapiens with and as ligands. The following page contains interesting information on the relation of 1D2S with [Anabolic Steroids]. Full crystallographic information is available from OCA.
Reference
Crystal structure of human sex hormone-binding globulin: steroid transport by a laminin G-like domain., Grishkovskaya I, Avvakumov GV, Sklenar G, Dales D, Hammond GL, Muller YA, EMBO J. 2000 Feb 15;19(4):504-12. PMID:10675319
Page seeded by OCA on Thu Feb 21 12:12:21 2008
