1d32
From Proteopedia
(New page: 200px<br /><applet load="1d32" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d32, resolution 1.700Å" /> '''DRUG-INDUCED DNA RE...) |
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| - | [[Image:1d32.jpg|left|200px]]<br /><applet load="1d32" size=" | + | [[Image:1d32.jpg|left|200px]]<br /><applet load="1d32" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d32, resolution 1.700Å" /> | caption="1d32, resolution 1.700Å" /> | ||
'''DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX'''<br /> | '''DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ditercalinium is a synthetic anticancer drug that binds to DNA by | + | Ditercalinium is a synthetic anticancer drug that binds to DNA by bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray crystallography, we have determined, to 1.7 A resolution, the three-dimensional structure of a complex of ditercalinium bound to the double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with ditercalinium is kinked (by 15 degrees) and severely unwound (by 36 degrees) with exceptionally wide major and minor grooves. Recognition of the DNA-ditercalinium complex by uvrABC in prokaryotes, and by mitochondrial DNA repair systems in eukaryotes, might be related to drug-induced distortion of the DNA helix. |
==About this Structure== | ==About this Structure== | ||
| - | 1D32 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with DIT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1D32 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=DIT:'>DIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D32 OCA]. |
==Reference== | ==Reference== | ||
Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex., Gao Q, Williams LD, Egli M, Rabinovich D, Chen SL, Quigley GJ, Rich A, Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2422-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2006181 2006181] | Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex., Gao Q, Williams LD, Egli M, Rabinovich D, Chen SL, Quigley GJ, Rich A, Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2422-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2006181 2006181] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chen, S | + | [[Category: Chen, S L.]] |
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Gao, Q.]] | [[Category: Gao, Q.]] | ||
| - | [[Category: Quigley, G | + | [[Category: Quigley, G J.]] |
[[Category: Rabinovich, D.]] | [[Category: Rabinovich, D.]] | ||
[[Category: Rich, A.]] | [[Category: Rich, A.]] | ||
| - | [[Category: Williams, L | + | [[Category: Williams, L D.]] |
[[Category: DIT]] | [[Category: DIT]] | ||
[[Category: complexed with drug]] | [[Category: complexed with drug]] | ||
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[[Category: right handed dna]] | [[Category: right handed dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:12:33 2008'' |
Revision as of 10:12, 21 February 2008
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DRUG-INDUCED DNA REPAIR: X-RAY STRUCTURE OF A DNA-DITERCALINIUM COMPLEX
Overview
Ditercalinium is a synthetic anticancer drug that binds to DNA by bis-intercalation and activates DNA repair processes. In prokaryotes, noncovalent DNA-ditercalinium complexes are incorrectly recognized by the uvrABC repair system as covalent lesions on DNA. In eukaryotes, mitochondrial DNA is degraded by excess and futile DNA repair. Using x-ray crystallography, we have determined, to 1.7 A resolution, the three-dimensional structure of a complex of ditercalinium bound to the double-stranded DNA fragment [d(CGCG)]2. The DNA in the complex with ditercalinium is kinked (by 15 degrees) and severely unwound (by 36 degrees) with exceptionally wide major and minor grooves. Recognition of the DNA-ditercalinium complex by uvrABC in prokaryotes, and by mitochondrial DNA repair systems in eukaryotes, might be related to drug-induced distortion of the DNA helix.
About this Structure
1D32 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Drug-induced DNA repair: X-ray structure of a DNA-ditercalinium complex., Gao Q, Williams LD, Egli M, Rabinovich D, Chen SL, Quigley GJ, Rich A, Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2422-6. PMID:2006181
Page seeded by OCA on Thu Feb 21 12:12:33 2008
