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1d5t
From Proteopedia
(New page: 200px<br /><applet load="1d5t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d5t, resolution 1.04Å" /> '''GUANINE NUCLEOTIDE D...) |
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| - | [[Image:1d5t.jpg|left|200px]]<br /><applet load="1d5t" size=" | + | [[Image:1d5t.jpg|left|200px]]<br /><applet load="1d5t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d5t, resolution 1.04Å" /> | caption="1d5t, resolution 1.04Å" /> | ||
'''GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM'''<br /> | '''GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that | + | Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine alpha-GDI at ultra-high resolution (1.04 A). Refinement at this resolution highlighted a region with high mobility of its main-chain residues. This corresponded to a surface loop in the primarily alpha-helical domain II at the base of alpha-GDI containing the previously uncharacterized sequence-conserved region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I at the apex of alpha-GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion. |
==About this Structure== | ==About this Structure== | ||
| - | 1D5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1D5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D5T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Balch, W | + | [[Category: Balch, W E.]] |
| - | [[Category: Greasley, S | + | [[Category: Greasley, S E.]] |
[[Category: Heine, A.]] | [[Category: Heine, A.]] | ||
[[Category: Kuhn, P.]] | [[Category: Kuhn, P.]] | ||
[[Category: Moyer, B.]] | [[Category: Moyer, B.]] | ||
[[Category: Peng, L.]] | [[Category: Peng, L.]] | ||
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: Zeng, K.]] | [[Category: Zeng, K.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: ultra-high resolution]] | [[Category: ultra-high resolution]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:15 2008'' |
Revision as of 10:13, 21 February 2008
|
GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM
Overview
Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine alpha-GDI at ultra-high resolution (1.04 A). Refinement at this resolution highlighted a region with high mobility of its main-chain residues. This corresponded to a surface loop in the primarily alpha-helical domain II at the base of alpha-GDI containing the previously uncharacterized sequence-conserved region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I at the apex of alpha-GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion.
About this Structure
1D5T is a Single protein structure of sequence from Bos taurus with as ligand. Full crystallographic information is available from OCA.
Reference
A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:11208110
Page seeded by OCA on Thu Feb 21 12:13:15 2008
Categories: Bos taurus | Single protein | Balch, W E. | Greasley, S E. | Heine, A. | Kuhn, P. | Moyer, B. | Peng, L. | Wilson, I A. | Zeng, K. | SO4 | Ultra-high resolution
