1d65
From Proteopedia
(New page: 200px<br /><applet load="1d65" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d65, resolution 2.200Å" /> '''MOLECULAR STRUCTURE...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1d65.gif|left|200px]]<br /><applet load="1d65" size=" | + | [[Image:1d65.gif|left|200px]]<br /><applet load="1d65" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d65, resolution 2.200Å" /> | caption="1d65, resolution 2.200Å" /> | ||
'''MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION'''<br /> | '''MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the dodecanucleotide duplex d(CGCAAATTTGCG)2 has | + | The crystal structure of the dodecanucleotide duplex d(CGCAAATTTGCG)2 has been solved to 2.2 A resolution and refined to an R-factor of 18.1% with the inclusion of 71 water molecules. The structure shows propeller twists of up to -20 degrees for the A.T base-pairs, although there is probably only one (weak) three-centre hydrogen bond in the six base-pair AT narrow minor-groove region. An extensive ribbon of hydration has been located in this groove that has features distinctive from the classic "spine of hydration". Solvation around phosphate groups is described, with several instances of water molecules bridging between phosphates. |
==About this Structure== | ==About this Structure== | ||
| - | 1D65 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1D65 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D65 OCA]. |
==Reference== | ==Reference== | ||
Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution., Edwards KJ, Brown DG, Spink N, Skelly JV, Neidle S, J Mol Biol. 1992 Aug 20;226(4):1161-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1518049 1518049] | Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution., Edwards KJ, Brown DG, Spink N, Skelly JV, Neidle S, J Mol Biol. 1992 Aug 20;226(4):1161-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1518049 1518049] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Brown, D | + | [[Category: Brown, D G.]] |
| - | [[Category: Edwards, K | + | [[Category: Edwards, K J.]] |
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: Spink, N.]] | [[Category: Spink, N.]] | ||
| Line 19: | Line 19: | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:20 2008'' |
Revision as of 10:13, 21 February 2008
|
MOLECULAR STRUCTURE OF THE B-DNA DODECAMER D(CGCAAATTTGCG)2; AN EXAMINATION OF PROPELLER TWIST AND MINOR-GROOVE WATER STRUCTURE AT 2.2 ANGSTROMS RESOLUTION
Overview
The crystal structure of the dodecanucleotide duplex d(CGCAAATTTGCG)2 has been solved to 2.2 A resolution and refined to an R-factor of 18.1% with the inclusion of 71 water molecules. The structure shows propeller twists of up to -20 degrees for the A.T base-pairs, although there is probably only one (weak) three-centre hydrogen bond in the six base-pair AT narrow minor-groove region. An extensive ribbon of hydration has been located in this groove that has features distinctive from the classic "spine of hydration". Solvation around phosphate groups is described, with several instances of water molecules bridging between phosphates.
About this Structure
1D65 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Molecular structure of the B-DNA dodecamer d(CGCAAATTTGCG)2. An examination of propeller twist and minor-groove water structure at 2.2 A resolution., Edwards KJ, Brown DG, Spink N, Skelly JV, Neidle S, J Mol Biol. 1992 Aug 20;226(4):1161-73. PMID:1518049
Page seeded by OCA on Thu Feb 21 12:13:20 2008
