1d64

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(New page: 200px<br /><applet load="1d64" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d64, resolution 2.100&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1d64.gif|left|200px]]<br /><applet load="1d64" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1d64.gif|left|200px]]<br /><applet load="1d64" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1d64, resolution 2.100&Aring;" />
caption="1d64, resolution 2.100&Aring;" />
'''CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES'''<br />
'''CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES'''<br />
==Overview==
==Overview==
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The crystal structure of the complex formed between the dodecanucleotide, d(CGCGAATTCGCG)2 and the drug pentamidine, which is active against the, Pneumocystis carinii pathogen in AIDS patients, has been determined to a, resolution of 2.1 A and an R-factor of 19.4%. Analysis of the structure, has shown the drug to be bound in the 5'-AATT minor groove region of the, duplex, with the amidinium groups H-bonded to adenine N3 atoms in an, interstrand manner. The drug molecule adopts an extended conformation, and, the immediate binding site spans four base pairs. Structural details of, the drug-DNA interactions are discussed, and comparison is made with the, dodecamer complex of the structurally similar berenil ligand.
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The crystal structure of the complex formed between the dodecanucleotide d(CGCGAATTCGCG)2 and the drug pentamidine, which is active against the Pneumocystis carinii pathogen in AIDS patients, has been determined to a resolution of 2.1 A and an R-factor of 19.4%. Analysis of the structure has shown the drug to be bound in the 5'-AATT minor groove region of the duplex, with the amidinium groups H-bonded to adenine N3 atoms in an interstrand manner. The drug molecule adopts an extended conformation, and the immediate binding site spans four base pairs. Structural details of the drug-DNA interactions are discussed, and comparison is made with the dodecamer complex of the structurally similar berenil ligand.
==About this Structure==
==About this Structure==
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1D64 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with PNT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D64 OCA].
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1D64 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PNT:'>PNT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D64 OCA].
==Reference==
==Reference==
Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1643044 1643044]
Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1643044 1643044]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Edwards, K.J.]]
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[[Category: Edwards, K J.]]
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[[Category: Jenkins, T.C.]]
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[[Category: Jenkins, T C.]]
[[Category: Neidle, S.]]
[[Category: Neidle, S.]]
[[Category: PNT]]
[[Category: PNT]]
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:34:42 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:23 2008''

Revision as of 10:13, 21 February 2008

Image:1d64.gif

1d64, resolution 2.100Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES

Overview

The crystal structure of the complex formed between the dodecanucleotide d(CGCGAATTCGCG)2 and the drug pentamidine, which is active against the Pneumocystis carinii pathogen in AIDS patients, has been determined to a resolution of 2.1 A and an R-factor of 19.4%. Analysis of the structure has shown the drug to be bound in the 5'-AATT minor groove region of the duplex, with the amidinium groups H-bonded to adenine N3 atoms in an interstrand manner. The drug molecule adopts an extended conformation, and the immediate binding site spans four base pairs. Structural details of the drug-DNA interactions are discussed, and comparison is made with the dodecamer complex of the structurally similar berenil ligand.

About this Structure

1D64 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:1643044

Page seeded by OCA on Thu Feb 21 12:13:23 2008

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