1pif
From Proteopedia
(New page: 200px<br /> <applet load="1pif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pif, resolution 2.3Å" /> '''PIG ALPHA-AMYLASE'''...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1PIF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PIF OCA]]. | + | 1PIF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Sites: AS, CA and CL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PIF OCA]]. |
==Reference== | ==Reference== | ||
Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their binding characteristics., Machius M, Vertesy L, Huber R, Wiegand G, J Mol Biol. 1996 Jul 19;260(3):409-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8757803 8757803] | Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their binding characteristics., Machius M, Vertesy L, Huber R, Wiegand G, J Mol Biol. 1996 Jul 19;260(3):409-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8757803 8757803] | ||
| + | [[Category: Alpha-amylase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
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[[Category: alpha-amylase alpha-1]] | [[Category: alpha-amylase alpha-1]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:49:43 2007'' |
Revision as of 11:45, 30 October 2007
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PIG ALPHA-AMYLASE
Overview
The crystal structures of porcine pancreatic alpha-amylase isozyme II (PPA, II) in its free form and complexed with the trestatin A derived, pseudo-octasaccharide V-1532 have been determined using Patterson search, techniques at resolutions of 2.3 and 2.2 angstroms, respectively. Seven, rings of the competitive inhibitor V-1532 could be detected in the active, site region as well as two maltose units in secondary binding sites on the, surface. V-1532 occupies the five central sugar binding subsites similar, to the PPA/acarbose structure. A sixth ring exists at the reducing end, connecting two symmetry related PPA molecules. The seventh moiety, a, 6-hydroxymethylconduritol ring, is located at the non-reducing end. The, electron density for this ring is relatively weak, indicating ... [(full description)]
About this Structure
1PIF is a [Single protein] structure of sequence from [Sus scrofa] with CA and CL as [ligands]. Active as [Alpha-amylase], with EC number [3.2.1.1]. Structure known Active Sites: AS, CA and CL. Full crystallographic information is available from [OCA].
Reference
Carbohydrate and protein-based inhibitors of porcine pancreatic alpha-amylase: structure analysis and comparison of their binding characteristics., Machius M, Vertesy L, Huber R, Wiegand G, J Mol Biol. 1996 Jul 19;260(3):409-21. PMID:8757803
Page seeded by OCA on Tue Oct 30 13:49:43 2007
