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1da1

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(New page: 200px<br /><applet load="1da1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1da1, resolution 2.250&Aring;" /> '''STRUCTURAL CHARACTE...)
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[[Image:1da1.gif|left|200px]]<br /><applet load="1da1" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1da1, resolution 2.250&Aring;" />
caption="1da1, resolution 2.250&Aring;" />
'''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT'''<br />
'''STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT'''<br />
==Overview==
==Overview==
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The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and, its structure analysed by X-ray diffraction. The unit cell, of dimensions, a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group, P2(1)2(1)2(1). The duplexes are in the Z conformation, with four, Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was, refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent, molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2, of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication, of the presence of ionised base pairs, in spite of the high pH of, crystallisation. The results are discussed in terms of the mutagenic, properties of 5- bromouracil.
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The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.
==About this Structure==
==About this Structure==
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1DA1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA].
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1DA1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA1 OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Brown, T.]]
[[Category: Brown, T.]]
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[[Category: Hunter, W.N.]]
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[[Category: Hunter, W N.]]
[[Category: Kennard, O.]]
[[Category: Kennard, O.]]
[[Category: Kneale, G.]]
[[Category: Kneale, G.]]
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[[Category: z-dna]]
[[Category: z-dna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:51:26 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:31 2008''

Revision as of 10:14, 21 February 2008

Image:1da1.gif

1da1, resolution 2.250Å

Drag the structure with the mouse to rotate

STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT

Overview

The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and its structure analysed by X-ray diffraction. The unit cell, of dimensions a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group P2(1)2(1)2(1). The duplexes are in the Z conformation, with four Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2 of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication of the presence of ionised base pairs, in spite of the high pH of crystallisation. The results are discussed in terms of the mutagenic properties of 5- bromouracil.

About this Structure

1DA1 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:3951996

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