1d9r
From Proteopedia
(New page: 200px<br /><applet load="1d9r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d9r, resolution 1.5Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1d9r.gif|left|200px]]<br /><applet load="1d9r" size=" | + | [[Image:1d9r.gif|left|200px]]<br /><applet load="1d9r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1d9r, resolution 1.5Å" /> | caption="1d9r, resolution 1.5Å" /> | ||
'''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS'''<br /> | '''CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | G x A mismatched base pairs are frequently found in nucleic acids. Human | + | G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general. |
==About this Structure== | ==About this Structure== | ||
| - | 1D9R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NCO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1D9R is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9R OCA]. |
==Reference== | ==Reference== | ||
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600106 10600106] | Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600106 10600106] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Gao, Y | + | [[Category: Gao, Y G.]] |
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
[[Category: Robinson, H.]] | [[Category: Robinson, H.]] | ||
[[Category: Sanishvili, R.]] | [[Category: Sanishvili, R.]] | ||
| - | [[Category: Wang, A | + | [[Category: Wang, A H.J.]] |
[[Category: NCO]] | [[Category: NCO]] | ||
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
| Line 22: | Line 22: | ||
[[Category: tandem ga base pairs]] | [[Category: tandem ga base pairs]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:32 2008'' |
Revision as of 10:14, 21 February 2008
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CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G-A BASE PAIRS
Overview
G x A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g. , (GAATG)n x (CATTC)n found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the "sheared" G x A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple-wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G x A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G x A base pairs in nucleic acids (including RNA, like ribozyme) in general.
About this Structure
1D9R is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure and recognition of sheared tandem G x A base pairs associated with human centromere DNA sequence at atomic resolution., Gao YG, Robinson H, Sanishvili R, Joachimiak A, Wang AH, Biochemistry. 1999 Dec 14;38(50):16452-60. PMID:10600106
Page seeded by OCA on Thu Feb 21 12:14:32 2008
