1daw
From Proteopedia
(New page: 200px<br /><applet load="1daw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1daw, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1daw.jpg|left|200px]]<br /><applet load="1daw" size=" | + | [[Image:1daw.jpg|left|200px]]<br /><applet load="1daw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1daw, resolution 2.2Å" /> | caption="1daw, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-AMPPNP'''<br /> | '''CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-AMPPNP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structures of the catalytic subunit of protein kinase CK2 from Zea | + | The structures of the catalytic subunit of protein kinase CK2 from Zea mays complexed with Mg2+ and with analogs of ATP or GTP were determined to 2.2 A resolution. Unlike most other protein kinases, CK2 from various sources shows 'dual-cosubstrate specificity', that is, the ability to efficiently use either ATP or GTP as a cosubstrate. The structures of these complexes demonstrate that water molecules are critical to switch the active site of CK2 from an ATP- to a GTP-compatible state. An understanding of the structural basis of dual-cosubstrate specificity may help in the design of drugs that target CK2 or other kinases with this property. |
==About this Structure== | ==About this Structure== | ||
| - | 1DAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with MG and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | + | 1DAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DAW OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Zea mays]] | [[Category: Zea mays]] | ||
[[Category: Guerra, B.]] | [[Category: Guerra, B.]] | ||
| - | [[Category: Issinger, O | + | [[Category: Issinger, O G.]] |
[[Category: Niefind, K.]] | [[Category: Niefind, K.]] | ||
[[Category: Puetter, M.]] | [[Category: Puetter, M.]] | ||
| Line 25: | Line 25: | ||
[[Category: protein kinase ck2]] | [[Category: protein kinase ck2]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:46 2008'' |
Revision as of 10:14, 21 February 2008
|
CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-AMPPNP
Overview
The structures of the catalytic subunit of protein kinase CK2 from Zea mays complexed with Mg2+ and with analogs of ATP or GTP were determined to 2.2 A resolution. Unlike most other protein kinases, CK2 from various sources shows 'dual-cosubstrate specificity', that is, the ability to efficiently use either ATP or GTP as a cosubstrate. The structures of these complexes demonstrate that water molecules are critical to switch the active site of CK2 from an ATP- to a GTP-compatible state. An understanding of the structural basis of dual-cosubstrate specificity may help in the design of drugs that target CK2 or other kinases with this property.
About this Structure
1DAW is a Single protein structure of sequence from Zea mays with and as ligands. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
GTP plus water mimic ATP in the active site of protein kinase CK2., Niefind K, Putter M, Guerra B, Issinger OG, Schomburg D, Nat Struct Biol. 1999 Dec;6(12):1100-3. PMID:10581548
Page seeded by OCA on Thu Feb 21 12:14:46 2008
