1dbq
From Proteopedia
(New page: 200px<br /><applet load="1dbq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dbq, resolution 2.2Å" /> '''DNA-BINDING REGULATOR...) |
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| - | [[Image:1dbq.jpg|left|200px]]<br /><applet load="1dbq" size=" | + | [[Image:1dbq.jpg|left|200px]]<br /><applet load="1dbq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dbq, resolution 2.2Å" /> | caption="1dbq, resolution 2.2Å" /> | ||
'''DNA-BINDING REGULATORY PROTEIN'''<br /> | '''DNA-BINDING REGULATORY PROTEIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The modulation of the affinity of DNA-binding proteins by small molecule | + | The modulation of the affinity of DNA-binding proteins by small molecule effectors for cognate DNA sites is common to both prokaryotes and eukaryotes. However, the mechanisms by which effector binding to one domain affects DNA binding by a distal domain are poorly understood structurally. In initial studies to provide insight into the mechanism of effector-modulated DNA binding of the lactose repressor family, we determined the crystal structure of the purine repressor bound to a corepressor and purF operator. To extend our understanding, we have determined the structure of the corepressor-free corepressor-binding domain of the purine repressor at 2.2 A resolution. In the unliganded state, structural changes in the corepressor-binding pocket cause each subunit to rotate open by as much as 23 degrees, the consequences of which are the disengagement of the minor groove-binding hinge helices and repressor-DNA dissociation. |
==About this Structure== | ==About this Structure== | ||
| - | 1DBQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DBQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brennan, R | + | [[Category: Brennan, R G.]] |
| - | [[Category: Choi, K | + | [[Category: Choi, K Y.]] |
[[Category: Lu, F.]] | [[Category: Lu, F.]] | ||
| - | [[Category: Schumacher, M | + | [[Category: Schumacher, M A.]] |
[[Category: Zalkin, H.]] | [[Category: Zalkin, H.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:56 2008'' |
Revision as of 10:14, 21 February 2008
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DNA-BINDING REGULATORY PROTEIN
Overview
The modulation of the affinity of DNA-binding proteins by small molecule effectors for cognate DNA sites is common to both prokaryotes and eukaryotes. However, the mechanisms by which effector binding to one domain affects DNA binding by a distal domain are poorly understood structurally. In initial studies to provide insight into the mechanism of effector-modulated DNA binding of the lactose repressor family, we determined the crystal structure of the purine repressor bound to a corepressor and purF operator. To extend our understanding, we have determined the structure of the corepressor-free corepressor-binding domain of the purine repressor at 2.2 A resolution. In the unliganded state, structural changes in the corepressor-binding pocket cause each subunit to rotate open by as much as 23 degrees, the consequences of which are the disengagement of the minor groove-binding hinge helices and repressor-DNA dissociation.
About this Structure
1DBQ is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
Mechanism of corepressor-mediated specific DNA binding by the purine repressor., Schumacher MA, Choi KY, Lu F, Zalkin H, Brennan RG, Cell. 1995 Oct 6;83(1):147-55. PMID:7553867
Page seeded by OCA on Thu Feb 21 12:14:56 2008
