1dg2
From Proteopedia
(New page: 200px<br /><applet load="1dg2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dg2" /> '''SOLUTION CONFORMATION OF A-CONOTOXIN AUIB'''...) |
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| - | [[Image:1dg2.gif|left|200px]]<br /><applet load="1dg2" size=" | + | [[Image:1dg2.gif|left|200px]]<br /><applet load="1dg2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dg2" /> | caption="1dg2" /> | ||
'''SOLUTION CONFORMATION OF A-CONOTOXIN AUIB'''<br /> | '''SOLUTION CONFORMATION OF A-CONOTOXIN AUIB'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The neuronal nicotinic acetylcholine receptors constitute a highly diverse | + | The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1DG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Conus aulicus]] | [[Category: Conus aulicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cho, J | + | [[Category: Cho, J H.]] |
| - | [[Category: Han, K | + | [[Category: Han, K H.]] |
| - | [[Category: McIntosh, J | + | [[Category: McIntosh, J M.]] |
| - | [[Category: Mok, K | + | [[Category: Mok, K H.]] |
| - | [[Category: Olivera, B | + | [[Category: Olivera, B M.]] |
| - | [[Category: Park, K | + | [[Category: Park, K H.]] |
[[Category: NH2]] | [[Category: NH2]] | ||
[[Category: a-helix]] | [[Category: a-helix]] | ||
[[Category: two disulfide bonds and c-term amidation]] | [[Category: two disulfide bonds and c-term amidation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:16:18 2008'' |
Revision as of 10:16, 21 February 2008
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SOLUTION CONFORMATION OF A-CONOTOXIN AUIB
Overview
The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition.
About this Structure
1DG2 is a Single protein structure of sequence from Conus aulicus with as ligand. Full crystallographic information is available from OCA.
Reference
Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist., Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH, J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709
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