1dl0
From Proteopedia
(New page: 200px<br /><applet load="1dl0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dl0" /> '''SOLUTION STRUCTURE OF THE INSECTICIDAL NEURO...) |
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| - | [[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size=" | + | [[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dl0" /> | caption="1dl0" /> | ||
'''SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C'''<br /> | '''SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have isolated a family of insect-selective neurotoxins from the venom | + | We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin. |
==About this Structure== | ==About this Structure== | ||
| - | 1DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. |
==Reference== | ==Reference== | ||
Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881200 10881200] | Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881200 10881200] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: King, G | + | [[Category: King, G F.]] |
| - | [[Category: Wang, X | + | [[Category: Wang, X H.]] |
[[Category: atracotoxin]] | [[Category: atracotoxin]] | ||
[[Category: cystine knot]] | [[Category: cystine knot]] | ||
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[[Category: vicinal disulfide]] | [[Category: vicinal disulfide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:43 2008'' |
Revision as of 10:17, 21 February 2008
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SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C
Overview
We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.
About this Structure
1DL0 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200
Page seeded by OCA on Thu Feb 21 12:17:43 2008
