1dm0
From Proteopedia
(New page: 200px<br /><applet load="1dm0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dm0, resolution 2.5Å" /> '''SHIGA TOXIN'''<br /> ...) |
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| - | [[Image:1dm0.gif|left|200px]]<br /><applet load="1dm0" size=" | + | [[Image:1dm0.gif|left|200px]]<br /><applet load="1dm0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dm0, resolution 2.5Å" /> | caption="1dm0, resolution 2.5Å" /> | ||
'''SHIGA TOXIN'''<br /> | '''SHIGA TOXIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Shigella dysenteriae is the pathogen responsible for the severe form of | + | Shigella dysenteriae is the pathogen responsible for the severe form of dysentery in humans. It produces Shiga toxin, the prototype of a family of closely related bacterial protein toxins. We have determined the structure of the holotoxin, an AB5 hexamer, by X-ray crystallography. The five B subunits form a pentameric ring, encircling a helix at the carboxy terminus of the A subunit. The A subunit interacts with the B pentamer via this C-terminal helix and a four-stranded mixed beta-sheet. The fold of the rest of the A subunit is similar to that of the A chain of the plant toxin ricin; both are N-glycosidases. However, the active site in the bacterial holotoxin is blocked by a segment of polypeptide chain. These residues of the A subunit would be released as part of the activation mechanism of the toxin. |
==About this Structure== | ==About this Structure== | ||
| - | 1DM0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Shigella_dysenteriae Shigella dysenteriae]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | + | 1DM0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Shigella_dysenteriae Shigella dysenteriae]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DM0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Shigella dysenteriae]] | [[Category: Shigella dysenteriae]] | ||
[[Category: rRNA N-glycosylase]] | [[Category: rRNA N-glycosylase]] | ||
| - | [[Category: Chernaia, M | + | [[Category: Chernaia, M M.]] |
| - | [[Category: Fraser, M | + | [[Category: Fraser, M E.]] |
| - | [[Category: James, M | + | [[Category: James, M N.]] |
| - | [[Category: Kozlov, Y | + | [[Category: Kozlov, Y V.]] |
[[Category: ab5 structure]] | [[Category: ab5 structure]] | ||
[[Category: active site]] | [[Category: active site]] | ||
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[[Category: polypeptide a]] | [[Category: polypeptide a]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:59 2008'' |
Revision as of 10:18, 21 February 2008
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SHIGA TOXIN
Overview
Shigella dysenteriae is the pathogen responsible for the severe form of dysentery in humans. It produces Shiga toxin, the prototype of a family of closely related bacterial protein toxins. We have determined the structure of the holotoxin, an AB5 hexamer, by X-ray crystallography. The five B subunits form a pentameric ring, encircling a helix at the carboxy terminus of the A subunit. The A subunit interacts with the B pentamer via this C-terminal helix and a four-stranded mixed beta-sheet. The fold of the rest of the A subunit is similar to that of the A chain of the plant toxin ricin; both are N-glycosidases. However, the active site in the bacterial holotoxin is blocked by a segment of polypeptide chain. These residues of the A subunit would be released as part of the activation mechanism of the toxin.
About this Structure
1DM0 is a Protein complex structure of sequences from Shigella dysenteriae. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
Reference
Crystal structure of the holotoxin from Shigella dysenteriae at 2.5 A resolution., Fraser ME, Chernaia MM, Kozlov YV, James MN, Nat Struct Biol. 1994 Jan;1(1):59-64. PMID:7656009
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