1dn4
From Proteopedia
(New page: 200px<br /><applet load="1dn4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dn4, resolution 1.600Å" /> '''SOLVATION OF THE LE...) |
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| - | [[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size=" | + | [[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dn4, resolution 1.600Å" /> | caption="1dn4, resolution 1.600Å" /> | ||
'''SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES'''<br /> | '''SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at | + | Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the absence of divalent cations. The crystals grown at 5 degrees C did not diffract X-rays, while those grown at 18 degrees C and 37 degrees C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal displays a spine of hydration running deep in the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings. In both crystalline forms, a hydrated sodium ion bound to the N-7 of a guanine ring was found. Strings of water molecules bridging phosphate anionic oxygen atoms are found along the backbone. The absolute values of the propeller-twist are also different in both structures although the values of the twist are very similar. The results point to the importance of the crystallization conditions when analysing fine structural details like solvation properties of oligomers. |
==About this Structure== | ==About this Structure== | ||
| - | 1DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chevrier, B.]] | [[Category: Chevrier, B.]] | ||
| - | [[Category: Dock, A | + | [[Category: Dock, A C.]] |
[[Category: Hartmann, B.]] | [[Category: Hartmann, B.]] | ||
[[Category: Leng, M.]] | [[Category: Leng, M.]] | ||
[[Category: Moras, D.]] | [[Category: Moras, D.]] | ||
| - | [[Category: Thuong, M | + | [[Category: Thuong, M T.]] |
[[Category: Westhof, E.]] | [[Category: Westhof, E.]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:21 2008'' |
Revision as of 10:18, 21 February 2008
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SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES
Overview
Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the absence of divalent cations. The crystals grown at 5 degrees C did not diffract X-rays, while those grown at 18 degrees C and 37 degrees C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal displays a spine of hydration running deep in the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings. In both crystalline forms, a hydrated sodium ion bound to the N-7 of a guanine ring was found. Strings of water molecules bridging phosphate anionic oxygen atoms are found along the backbone. The absolute values of the propeller-twist are also different in both structures although the values of the twist are very similar. The results point to the importance of the crystallization conditions when analysing fine structural details like solvation properties of oligomers.
About this Structure
1DN4 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solvation of the left-handed hexamer d(5BrC-G-5BrC-G-5 BrC-G) in crystals grown at two temperatures., Chevrier B, Dock AC, Hartmann B, Leng M, Moras D, Thuong MT, Westhof E, J Mol Biol. 1986 Apr 20;188(4):707-19. PMID:3735433
Page seeded by OCA on Thu Feb 21 12:18:21 2008
Categories: Protein complex | Chevrier, B. | Dock, A C. | Hartmann, B. | Leng, M. | Moras, D. | Thuong, M T. | Westhof, E. | Double helix | Modified | Z-dna
