1dpn
From Proteopedia
(New page: 200px<br /><applet load="1dpn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dpn, resolution 0.95Å" /> '''B-DODECAMER CGCGAA(T...) |
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| - | [[Image:1dpn.gif|left|200px]]<br /><applet load="1dpn" size=" | + | [[Image:1dpn.gif|left|200px]]<br /><applet load="1dpn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dpn, resolution 0.95Å" /> | caption="1dpn, resolution 0.95Å" /> | ||
'''B-DODECAMER CGCGAA(TAF)TCGCG WITH INCORPORATED 2'-DEOXY-2'-FLUORO-ARABINO-FURANOSYL THYMINE'''<br /> | '''B-DODECAMER CGCGAA(TAF)TCGCG WITH INCORPORATED 2'-DEOXY-2'-FLUORO-ARABINO-FURANOSYL THYMINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined single crystal structures of an A-DNA decamer and a | + | We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition. |
==About this Structure== | ==About this Structure== | ||
| - | 1DPN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DPN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: An, H.]] | [[Category: An, H.]] | ||
| - | [[Category: Cook, P | + | [[Category: Cook, P Dan.]] |
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
| - | [[Category: Maier, M | + | [[Category: Maier, M A.]] |
[[Category: Manoharan, M.]] | [[Category: Manoharan, M.]] | ||
[[Category: Miller, R.]] | [[Category: Miller, R.]] | ||
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[[Category: Teplova, M.]] | [[Category: Teplova, M.]] | ||
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
| - | [[Category: Weeks, C | + | [[Category: Weeks, C M.]] |
[[Category: MG]] | [[Category: MG]] | ||
| - | [[Category: 0 | + | [[Category: 0 95 a resolution structure]] |
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:19:04 2008'' |
Revision as of 10:19, 21 February 2008
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B-DODECAMER CGCGAA(TAF)TCGCG WITH INCORPORATED 2'-DEOXY-2'-FLUORO-ARABINO-FURANOSYL THYMINE
Overview
We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.
About this Structure
1DPN is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution., Egli M, Tereshko V, Teplova M, Minasov G, Joachimiak A, Sanishvili R, Weeks CM, Miller R, Maier MA, An H, Dan Cook P, Manoharan M, Biopolymers. 1998;48(4):234-52. PMID:10699842
Page seeded by OCA on Thu Feb 21 12:19:04 2008
