1dqy
From Proteopedia
(New page: 200px<br /><applet load="1dqy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dqy, resolution 1.83Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1dqy.gif|left|200px]]<br /><applet load="1dqy" size=" | + | [[Image:1dqy.gif|left|200px]]<br /><applet load="1dqy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dqy, resolution 1.83Å" /> | caption="1dqy, resolution 1.83Å" /> | ||
'''CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS WITH DIETHYL PHOSPHATE INHIBITOR'''<br /> | '''CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS WITH DIETHYL PHOSPHATE INHIBITOR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The antigen 85 (ag85) complex, composed of three proteins (ag85A, B and | + | The antigen 85 (ag85) complex, composed of three proteins (ag85A, B and C), is a major protein component of the Mycobacterium tuberculosis cell wall. Each protein possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. The crystal structure of recombinant ag85C from M. tuberculosis, refined to a resolution of 1.5 A, reveals an alpha/beta-hydrolase polypeptide fold, and a catalytic triad formed by Ser 124, Glu 228 and His 260. ag85C complexed with a covalent inhibitor implicates residues Leu 40 and Met 125 as components of the oxyanion hole. A hydrophobic pocket and tunnel extending 21 A into the core of the protein indicates the location of a probable trehalose monomycolate binding site. Also, a large region of conserved surface residues among ag85A, B and C is a probable site for the interaction of ag85 proteins with human fibronectin. |
==About this Structure== | ==About this Structure== | ||
| - | 1DQY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with DEP and MRD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1DQY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=DEP:'>DEP</scene> and <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Klabunde, T.]] | [[Category: Klabunde, T.]] | ||
| - | [[Category: Ronning, D | + | [[Category: Ronning, D R.]] |
| - | [[Category: Sacchettini, J | + | [[Category: Sacchettini, J C.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
[[Category: DEP]] | [[Category: DEP]] | ||
[[Category: MRD]] | [[Category: MRD]] | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:19:31 2008'' |
Revision as of 10:19, 21 February 2008
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CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS WITH DIETHYL PHOSPHATE INHIBITOR
Overview
The antigen 85 (ag85) complex, composed of three proteins (ag85A, B and C), is a major protein component of the Mycobacterium tuberculosis cell wall. Each protein possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. The crystal structure of recombinant ag85C from M. tuberculosis, refined to a resolution of 1.5 A, reveals an alpha/beta-hydrolase polypeptide fold, and a catalytic triad formed by Ser 124, Glu 228 and His 260. ag85C complexed with a covalent inhibitor implicates residues Leu 40 and Met 125 as components of the oxyanion hole. A hydrophobic pocket and tunnel extending 21 A into the core of the protein indicates the location of a probable trehalose monomycolate binding site. Also, a large region of conserved surface residues among ag85A, B and C is a probable site for the interaction of ag85 proteins with human fibronectin.
About this Structure
1DQY is a Single protein structure of sequence from Mycobacterium tuberculosis with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines., Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC, Nat Struct Biol. 2000 Feb;7(2):141-6. PMID:10655617
Page seeded by OCA on Thu Feb 21 12:19:31 2008
Categories: Mycobacterium tuberculosis | Single protein | Klabunde, T. | Ronning, D R. | Sacchettini, J C. | TBSGC, TB Structural Genomics Consortium. | DEP | MRD | 85c | Antigen | Dpi | Fibronectin | Protein structure initiative | Psi | Structural genomics | Tb structural genomics consortium | Tbsgc
