1dsz
From Proteopedia
(New page: 200px<br /> <applet load="1dsz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dsz, resolution 1.70Å" /> '''STRUCTURE OF THE RX...) |
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| - | [[Image:1dsz.gif|left|200px]]<br /> | + | [[Image:1dsz.gif|left|200px]]<br /><applet load="1dsz" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1dsz, resolution 1.70Å" /> | caption="1dsz, resolution 1.70Å" /> | ||
'''STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1'''<br /> | '''STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 9-cis retinoic acid receptor (retinoid X receptor, RXR) forms | + | The 9-cis retinoic acid receptor (retinoid X receptor, RXR) forms heterodimers with the all-trans retinoic acid receptor (RAR) and other nuclear receptors on DNA regulatory sites composed of tandem binding elements. We describe the 1.70 A resolution structure of the ternary complex of RXR and RAR DNA-binding regions in complex with the retinoic acid response element DR1. The receptors recognize identical half-sites through extensive base-specific contacts; however, RXR binds exclusively to the 3' site to form an asymmetric complex with the reverse polarity of other RXR heterodimers. The subunits associate in a strictly DNA-dependent manner using the T-box of RXR and the Zn-II region of RAR, both of which are reshaped in forming the complex. The protein-DNA contacts, the dimerization interface and the DNA curvature in the RXR-RAR complex are distinct from those of the RXR homodimer, which also binds DR1. Together, these structures illustrate how the nuclear receptor superfamily exploits conformational flexibility and locally induced structures to generate combinatorial transcription factors. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DSZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DSZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rxr]] | [[Category: rxr]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:09 2008'' |
Revision as of 10:20, 21 February 2008
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STRUCTURE OF THE RXR/RAR DNA-BINDING DOMAIN HETERODIMER IN COMPLEX WITH THE RETINOIC ACID RESPONSE ELEMENT DR1
Contents |
Overview
The 9-cis retinoic acid receptor (retinoid X receptor, RXR) forms heterodimers with the all-trans retinoic acid receptor (RAR) and other nuclear receptors on DNA regulatory sites composed of tandem binding elements. We describe the 1.70 A resolution structure of the ternary complex of RXR and RAR DNA-binding regions in complex with the retinoic acid response element DR1. The receptors recognize identical half-sites through extensive base-specific contacts; however, RXR binds exclusively to the 3' site to form an asymmetric complex with the reverse polarity of other RXR heterodimers. The subunits associate in a strictly DNA-dependent manner using the T-box of RXR and the Zn-II region of RAR, both of which are reshaped in forming the complex. The protein-DNA contacts, the dimerization interface and the DNA curvature in the RXR-RAR complex are distinct from those of the RXR homodimer, which also binds DR1. Together, these structures illustrate how the nuclear receptor superfamily exploits conformational flexibility and locally induced structures to generate combinatorial transcription factors.
Disease
Known disease associated with this structure: Leukemia, acute promyelocytic OMIM:[180240]
About this Structure
1DSZ is a Protein complex structure of sequences from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the RXR-RAR DNA-binding complex on the retinoic acid response element DR1., Rastinejad F, Wagner T, Zhao Q, Khorasanizadeh S, EMBO J. 2000 Mar 1;19(5):1045-54. PMID:10698945
Page seeded by OCA on Thu Feb 21 12:20:09 2008
Categories: Homo sapiens | Protein complex | Khorasanizadeh, S. | Rastinejad, F. | Wagner, T. | Zhao, Q. | ZN | Nuclear receptor | Protein-dna | Rar | Retinoic acid | Rxr
