1duq
From Proteopedia
(New page: 200px<br /> <applet load="1duq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1duq, resolution 2.10Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1duq.gif|left|200px]]<br /> | + | [[Image:1duq.gif|left|200px]]<br /><applet load="1duq" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1duq" size=" | + | |
caption="1duq, resolution 2.10Å" /> | caption="1duq, resolution 2.10Å" /> | ||
'''CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1'''<br /> | '''CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal and molecular structure of an RNA duplex corresponding to the | + | The crystal and molecular structure of an RNA duplex corresponding to the high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution. Four unique duplexes are present in the crystal, comprising two structural variants. In each duplex, the RNA double helix consists of an annealed 12-mer and 14-mer that form an asymmetric internal loop consisting of G-G and G-A noncanonical base pairs and a flipped-out uridine. The 12-mer strand has an A-form conformation, whereas the 14-mer strand is distorted to accommodate the bulges and noncanonical base pairing. In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydrogen bonding, is formed in each helix. The G-A base pairing agrees with the NMR structure in one structural variant, but forms a novel water-mediated pair in the other. A backbone flip and reorientation of the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex between the RBE and the Rev peptide. |
==About this Structure== | ==About this Structure== | ||
| - | 1DUQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1DUQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DUQ OCA]. |
==Reference== | ==Reference== | ||
The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability., Hung LW, Holbrook EL, Holbrook SR, Proc Natl Acad Sci U S A. 2000 May 9;97(10):5107-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10792052 10792052] | The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability., Hung LW, Holbrook EL, Holbrook SR, Proc Natl Acad Sci U S A. 2000 May 9;97(10):5107-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10792052 10792052] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Holbrook, E | + | [[Category: Holbrook, E L.]] |
| - | [[Category: Holbrook, S | + | [[Category: Holbrook, S R.]] |
| - | [[Category: Hung, L | + | [[Category: Hung, L W.]] |
[[Category: NA]] | [[Category: NA]] | ||
[[Category: hiv-1]] | [[Category: hiv-1]] | ||
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[[Category: rre]] | [[Category: rre]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:20:43 2008'' |
Revision as of 10:20, 21 February 2008
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CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1
Overview
The crystal and molecular structure of an RNA duplex corresponding to the high affinity Rev protein binding element (RBE) has been determined at 2.1-A resolution. Four unique duplexes are present in the crystal, comprising two structural variants. In each duplex, the RNA double helix consists of an annealed 12-mer and 14-mer that form an asymmetric internal loop consisting of G-G and G-A noncanonical base pairs and a flipped-out uridine. The 12-mer strand has an A-form conformation, whereas the 14-mer strand is distorted to accommodate the bulges and noncanonical base pairing. In contrast to the NMR model of the unbound RBE, an asymmetric G-G pair with N2-N7 and N1-O6 hydrogen bonding, is formed in each helix. The G-A base pairing agrees with the NMR structure in one structural variant, but forms a novel water-mediated pair in the other. A backbone flip and reorientation of the G-G base pair is required to assume the RBE conformation present in the NMR model of the complex between the RBE and the Rev peptide.
About this Structure
1DUQ is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of the Rev binding element of HIV-1 reveals novel base pairing and conformational variability., Hung LW, Holbrook EL, Holbrook SR, Proc Natl Acad Sci U S A. 2000 May 9;97(10):5107-12. PMID:10792052
Page seeded by OCA on Thu Feb 21 12:20:43 2008
Categories: Protein complex | Holbrook, E L. | Holbrook, S R. | Hung, L W. | NA | Hiv-1 | Rev binding domain | Rre
