1dvp
From Proteopedia
(New page: 200px<br /><applet load="1dvp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dvp, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1dvp.gif|left|200px]]<br /><applet load="1dvp" size=" | + | [[Image:1dvp.gif|left|200px]]<br /><applet load="1dvp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dvp, resolution 2.0Å" /> | caption="1dvp, resolution 2.0Å" /> | ||
'''CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION'''<br /> | '''CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the 2 A X-ray structure of the 219-residue N-terminal | + | We have determined the 2 A X-ray structure of the 219-residue N-terminal VHS and FYVE tandem domain unit of Drosophila Hrs. The unit assumes a pyramidal structure in which the much larger VHS domain (residues 1-153) forms a rectangular base and the FYVE domain occupies the apical end. The VHS domain is comprised of an unusual "superhelix" of eight alpha helices, and the FYVE domain is mainly built of loops, two double-stranded antiparallel sheets, and a helix stabilized by two tetrahedrally coordinated zinc atoms. The two-domain structure forms an exact 2-fold-related homodimer through antiparallel association of mainly FYVE domains. Dimerization creates two identical pockets designed for binding ligands with multiple negative charges such as citrate or phosphatidylinositol 3-phosphate. |
==About this Structure== | ==About this Structure== | ||
| - | 1DVP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN and CIT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1DVP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bellen, H.]] | [[Category: Bellen, H.]] | ||
[[Category: Duan, X.]] | [[Category: Duan, X.]] | ||
| - | [[Category: Lloyd, T | + | [[Category: Lloyd, T E.]] |
[[Category: Mao, Y.]] | [[Category: Mao, Y.]] | ||
[[Category: Nickitenko, A.]] | [[Category: Nickitenko, A.]] | ||
| - | [[Category: Quiocho, F | + | [[Category: Quiocho, F A.]] |
| - | [[Category: Wu, M | + | [[Category: Wu, M N.]] |
[[Category: CIT]] | [[Category: CIT]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:21:01 2008'' |
Revision as of 10:21, 21 February 2008
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CRYSTAL STRUCTURE OF THE VHS AND FYVE TANDEM DOMAINS OF HRS, A PROTEIN INVOLVED IN MEMBRANE TRAFFICKING AND SIGNAL TRANSDUCTION
Overview
We have determined the 2 A X-ray structure of the 219-residue N-terminal VHS and FYVE tandem domain unit of Drosophila Hrs. The unit assumes a pyramidal structure in which the much larger VHS domain (residues 1-153) forms a rectangular base and the FYVE domain occupies the apical end. The VHS domain is comprised of an unusual "superhelix" of eight alpha helices, and the FYVE domain is mainly built of loops, two double-stranded antiparallel sheets, and a helix stabilized by two tetrahedrally coordinated zinc atoms. The two-domain structure forms an exact 2-fold-related homodimer through antiparallel association of mainly FYVE domains. Dimerization creates two identical pockets designed for binding ligands with multiple negative charges such as citrate or phosphatidylinositol 3-phosphate.
About this Structure
1DVP is a Single protein structure of sequence from Drosophila melanogaster with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the VHS and FYVE tandem domains of Hrs, a protein involved in membrane trafficking and signal transduction., Mao Y, Nickitenko A, Duan X, Lloyd TE, Wu MN, Bellen H, Quiocho FA, Cell. 2000 Feb 18;100(4):447-56. PMID:10693761
Page seeded by OCA on Thu Feb 21 12:21:01 2008
Categories: Drosophila melanogaster | Single protein | Bellen, H. | Duan, X. | Lloyd, T E. | Mao, Y. | Nickitenko, A. | Quiocho, F A. | Wu, M N. | CIT | ZN | Fyve | Hrs | Superhelix | Vhs | Zinc finger
