1dzt
From Proteopedia
(New page: 200px<br /><applet load="1dzt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dzt, resolution 2.2Å" /> '''RMLC FROM SALMONELLA ...) |
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| - | [[Image:1dzt.jpg|left|200px]]<br /><applet load="1dzt" size=" | + | [[Image:1dzt.jpg|left|200px]]<br /><applet load="1dzt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dzt, resolution 2.2Å" /> | caption="1dzt, resolution 2.2Å" /> | ||
'''RMLC FROM SALMONELLA TYPHIMURIUM'''<br /> | '''RMLC FROM SALMONELLA TYPHIMURIUM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Deoxythymidine diphosphate (dTDP)-L-rhamnose is the precursor of | + | Deoxythymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose, a saccharide required for the virulence of some pathogenic bacteria. dTDP-L-rhamnose is synthesized from glucose-1-phosphate and deoxythymidine triphosphate (dTTP) via a pathway involving four distinct enzymes. This pathway does not exist in humans and the enzymes involved in dTDP-L-rhamnose synthesis are potential targets for the design of new therapeutic agents. Here, the crystal structure of dTDP-6-deoxy-D-xylo-4-hexulose 3,5 epimerase (RmlC, EC5.1.3.13) from Salmonella enterica serovar Typhimurium was determined. The third enzyme of the rhamnose biosynthetic pathway, RmlC epimerizes at two carbon centers, the 3 and 5 positions of the sugar ring. The structure was determined by multiwavelength anomalous diffraction to a resolution of 2.17 A. RmlC is a dimer and each monomer is formed mainly from two beta-sheets arranged in a beta-sandwich. The structure of a dTDP-phenol-RmlC complex shows the substrate-binding site to be located between the two beta-sheets; this site is formed from residues of both monomers. Sequence alignments of other RmlC enzymes confirm that this region is very highly conserved. The enzyme is distinct structurally from other epimerases known and thus, is the first example of a new class of carbohydrate epimerase. |
==About this Structure== | ==About this Structure== | ||
| - | 1DZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with SO4, TPE, ATY and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/dTDP-4-dehydrorhamnose_3,5-epimerase dTDP-4-dehydrorhamnose 3,5-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.13 5.1.3.13] Full crystallographic information is available from [http:// | + | 1DZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=TPE:'>TPE</scene>, <scene name='pdbligand=ATY:'>ATY</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/dTDP-4-dehydrorhamnose_3,5-epimerase dTDP-4-dehydrorhamnose 3,5-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.13 5.1.3.13] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: dTDP-4-dehydrorhamnose 3,5-epimerase]] | [[Category: dTDP-4-dehydrorhamnose 3,5-epimerase]] | ||
| - | [[Category: Giraud, M | + | [[Category: Giraud, M F.]] |
| - | [[Category: Naismith, J | + | [[Category: Naismith, J H.]] |
[[Category: ATY]] | [[Category: ATY]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: 5 hexulose epimerase]] | [[Category: 5 hexulose epimerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:13 2008'' |
Revision as of 10:22, 21 February 2008
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RMLC FROM SALMONELLA TYPHIMURIUM
Overview
Deoxythymidine diphosphate (dTDP)-L-rhamnose is the precursor of L-rhamnose, a saccharide required for the virulence of some pathogenic bacteria. dTDP-L-rhamnose is synthesized from glucose-1-phosphate and deoxythymidine triphosphate (dTTP) via a pathway involving four distinct enzymes. This pathway does not exist in humans and the enzymes involved in dTDP-L-rhamnose synthesis are potential targets for the design of new therapeutic agents. Here, the crystal structure of dTDP-6-deoxy-D-xylo-4-hexulose 3,5 epimerase (RmlC, EC5.1.3.13) from Salmonella enterica serovar Typhimurium was determined. The third enzyme of the rhamnose biosynthetic pathway, RmlC epimerizes at two carbon centers, the 3 and 5 positions of the sugar ring. The structure was determined by multiwavelength anomalous diffraction to a resolution of 2.17 A. RmlC is a dimer and each monomer is formed mainly from two beta-sheets arranged in a beta-sandwich. The structure of a dTDP-phenol-RmlC complex shows the substrate-binding site to be located between the two beta-sheets; this site is formed from residues of both monomers. Sequence alignments of other RmlC enzymes confirm that this region is very highly conserved. The enzyme is distinct structurally from other epimerases known and thus, is the first example of a new class of carbohydrate epimerase.
About this Structure
1DZT is a Single protein structure of sequence from Salmonella typhimurium with , , and as ligands. Active as dTDP-4-dehydrorhamnose 3,5-epimerase, with EC number 5.1.3.13 Full crystallographic information is available from OCA.
Reference
RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase., Giraud MF, Leonard GA, Field RA, Berlind C, Naismith JH, Nat Struct Biol. 2000 May;7(5):398-402. PMID:10802738
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