1dzf
From Proteopedia
(New page: 200px<br /><applet load="1dzf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dzf, resolution 1.9Å" /> '''RPB5 FROM S.CEREVISIA...) |
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| - | [[Image:1dzf.jpg|left|200px]]<br /><applet load="1dzf" size=" | + | [[Image:1dzf.jpg|left|200px]]<br /><applet load="1dzf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dzf, resolution 1.9Å" /> | caption="1dzf, resolution 1.9Å" /> | ||
'''RPB5 FROM S.CEREVISIAE'''<br /> | '''RPB5 FROM S.CEREVISIAE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Eukaryotic nuclei contain three different types of RNA polymerases | + | Eukaryotic nuclei contain three different types of RNA polymerases (RNAPs), each consisting of 12-18 different subunits. The evolutionarily highly conserved RNAP subunit RPB5 is shared by all three enzymes and therefore represents a key structural/functional component of all eukaryotic RNAPs. Here we present the crystal structure of the RPB5 subunit from Saccharomyces cerevisiae. The bipartite structure includes a eukaryote-specific N-terminal domain and a C-terminal domain resembling the archaeal RNAP subunit H. RPB5 has been implicated in direct protein-protein contacts with transcription factor IIB, one of the components of the RNAP(II) basal transcriptional machinery, and gene-specific activator proteins, such as the hepatitis B virus transactivator protein X. The experimentally mapped regions of RPB5 involved in these interactions correspond to distinct and surface-exposed alpha-helical structures. |
==About this Structure== | ==About this Structure== | ||
| - | 1DZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http:// | + | 1DZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Onesti, S.]] | [[Category: Onesti, S.]] | ||
[[Category: Todone, F.]] | [[Category: Todone, F.]] | ||
| - | [[Category: Weinzierl, R | + | [[Category: Weinzierl, R O.J.]] |
[[Category: rna polymerase subunit]] | [[Category: rna polymerase subunit]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:15 2008'' |
Revision as of 10:22, 21 February 2008
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RPB5 FROM S.CEREVISIAE
Overview
Eukaryotic nuclei contain three different types of RNA polymerases (RNAPs), each consisting of 12-18 different subunits. The evolutionarily highly conserved RNAP subunit RPB5 is shared by all three enzymes and therefore represents a key structural/functional component of all eukaryotic RNAPs. Here we present the crystal structure of the RPB5 subunit from Saccharomyces cerevisiae. The bipartite structure includes a eukaryote-specific N-terminal domain and a C-terminal domain resembling the archaeal RNAP subunit H. RPB5 has been implicated in direct protein-protein contacts with transcription factor IIB, one of the components of the RNAP(II) basal transcriptional machinery, and gene-specific activator proteins, such as the hepatitis B virus transactivator protein X. The experimentally mapped regions of RPB5 involved in these interactions correspond to distinct and surface-exposed alpha-helical structures.
About this Structure
1DZF is a Single protein structure of sequence from Saccharomyces cerevisiae. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Full crystallographic information is available from OCA.
Reference
Crystal structure of RPB5, a universal eukaryotic RNA polymerase subunit and transcription factor interaction target., Todone F, Weinzierl RO, Brick P, Onesti S, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6306-10. PMID:10841537
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