1e07
From Proteopedia
(New page: 200px<br /> <applet load="1e07" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e07" /> '''THEORETICAL MODEL OF HUMAN CARCINOEMBRYONIC...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1e07.gif|left|200px]]<br /> | + | [[Image:1e07.gif|left|200px]]<br /><applet load="1e07" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e07" size=" | + | |
caption="1e07" /> | caption="1e07" /> | ||
'''THEORETICAL MODEL OF HUMAN CARCINOEMBRYONIC ANTIGEN BY HOMOLOGY MODELLING AND CURVE-FITTING TO EXPERIMENTAL SOLUTION SCATTERING DATA'''<br /> | '''THEORETICAL MODEL OF HUMAN CARCINOEMBRYONIC ANTIGEN BY HOMOLOGY MODELLING AND CURVE-FITTING TO EXPERIMENTAL SOLUTION SCATTERING DATA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | MFE23 is a single chain Fv antibody that has a high affinity for | + | MFE23 is a single chain Fv antibody that has a high affinity for carcinoembryonic antigen (CEA). A full homology model for CEA based on V-type, I-type and C2-type immunoglobulin folds, 28 oligosaccharides and the interdomain angle of CD2 was validated using solution scattering data. The superimposition of the intermolecular contacts observed in our recent crystal structure of MFE23 with the N-terminal domain of CEA permitted the MFE23-CEA complex to be modelled. Good surface and electrostatic complementarity and carbohydrate-unhindered access of MFE23 with the indentation between the first two CEA domains was observed. The model is supported by biochemical data and provides insight on the high affinity of MFE23 for CEA. |
==About this Structure== | ==About this Structure== | ||
| - | 1E07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1E07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E07 OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 13: | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Boehm, M | + | [[Category: Boehm, M K.]] |
| - | [[Category: Perkins, S | + | [[Category: Perkins, S J.]] |
[[Category: calpha only]] | [[Category: calpha only]] | ||
[[Category: cea]] | [[Category: cea]] | ||
| Line 21: | Line 20: | ||
[[Category: tumour marker]] | [[Category: tumour marker]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:30 2008'' |
Revision as of 10:22, 21 February 2008
|
THEORETICAL MODEL OF HUMAN CARCINOEMBRYONIC ANTIGEN BY HOMOLOGY MODELLING AND CURVE-FITTING TO EXPERIMENTAL SOLUTION SCATTERING DATA
Overview
MFE23 is a single chain Fv antibody that has a high affinity for carcinoembryonic antigen (CEA). A full homology model for CEA based on V-type, I-type and C2-type immunoglobulin folds, 28 oligosaccharides and the interdomain angle of CD2 was validated using solution scattering data. The superimposition of the intermolecular contacts observed in our recent crystal structure of MFE23 with the N-terminal domain of CEA permitted the MFE23-CEA complex to be modelled. Good surface and electrostatic complementarity and carbohydrate-unhindered access of MFE23 with the indentation between the first two CEA domains was observed. The model is supported by biochemical data and provides insight on the high affinity of MFE23 for CEA.
About this Structure
1E07 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23., Boehm MK, Perkins SJ, FEBS Lett. 2000 Jun 9;475(1):11-6. PMID:10854848
Page seeded by OCA on Thu Feb 21 12:22:30 2008
