1e4p
From Proteopedia
(New page: 200px<br /><applet load="1e4p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e4p" /> '''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN ...) |
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'''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''<br /> | '''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The cleavage site of the Neurospora VS RNA ribozyme is located in a | + | The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1E4P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1E4P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA]. |
==Reference== | ==Reference== | ||
Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11142381 11142381] | Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11142381 11142381] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Heus, H | + | [[Category: Heus, H A.]] |
| - | [[Category: Hilbers, C | + | [[Category: Hilbers, C W.]] |
| - | [[Category: Michiels, P | + | [[Category: Michiels, P J.A.]] |
| - | [[Category: Schouten, C | + | [[Category: Schouten, C H.J.]] |
[[Category: rna ribozyme]] | [[Category: rna ribozyme]] | ||
[[Category: substrate hairpin]] | [[Category: substrate hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:23:48 2008'' |
Revision as of 10:23, 21 February 2008
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STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE
Overview
The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
About this Structure
1E4P is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381
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