1ogz
From Proteopedia
(New page: 200px<br /> <applet load="1ogz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ogz, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1OGZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]] with EQU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGZ OCA]]. | + | 1OGZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]] with EQU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]]. Structure known Active Site: EQU. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OGZ OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: Comamonas testosteroni]] | [[Category: Comamonas testosteroni]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| + | [[Category: Steroid Delta-isomerase]] | ||
[[Category: Cha, S.S.]] | [[Category: Cha, S.S.]] | ||
[[Category: Choi, K.Y.]] | [[Category: Choi, K.Y.]] | ||
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[[Category: ketosteroid]] | [[Category: ketosteroid]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:54:44 2007'' |
Revision as of 11:50, 30 October 2007
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CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANTS P39A COMPLEXED WITH EQUILENIN FROM PSEUDOMONAS TESTOSTERONI
Overview
KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric, enzyme that catalyses the allylic isomerization of Delta5-3-ketosteroids, to their conjugated Delta4-isomers at a reaction rate equivalent to the, diffusion-controlled limit. Based on the structural analysis of KSI at a, high resolution, the conserved cis-Pro39 residue was proposed to be, involved in the proper positioning of Asp38, a critical catalytic residue, since the residue was found not only to be structurally associated with, Asp38, but also to confer a structural rigidity on the local active-site, geometry consisting of Asp38, Pro39, Val40, Gly41 and Ser42 at the, flexible loop between b-strands B1 and B2. In order to investigate the, structural role of the conserved cis-Pro39 residue near the active site ... [(full description)]
About this Structure
1OGZ is a [Single protein] structure of sequence from [Comamonas testosteroni] with EQU as [ligand]. Active as [Steroid Delta-isomerase], with EC number [5.3.3.1]. Structure known Active Site: EQU. Full crystallographic information is available from [OCA].
Reference
The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni., Nam GH, Cha SS, Yun YS, Oh YH, Hong BH, Lee HS, Choi KY, Biochem J. 2003 Oct 15;375(Pt 2):297-305. PMID:12852789
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