1e7k
From Proteopedia
(New page: 200px<br /> <applet load="1e7k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e7k, resolution 2.9Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1e7k.gif|left|200px]]<br /> | + | [[Image:1e7k.gif|left|200px]]<br /><applet load="1e7k" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1e7k" size=" | + | |
caption="1e7k, resolution 2.9Å" /> | caption="1e7k, resolution 2.9Å" /> | ||
'''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT'''<br /> | '''CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the crystal structure of a spliceosomal RNP complex | + | We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1E7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E7K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: u4 snrna]] | [[Category: u4 snrna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:41 2008'' |
Revision as of 10:24, 21 February 2008
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CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT
Overview
We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
About this Structure
1E7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment., Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R, Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207
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