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1e9i
From Proteopedia
(New page: 200px<br /><applet load="1e9i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e9i, resolution 2.48Å" /> '''ENOLASE FROM E.COLI'...) |
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| - | [[Image:1e9i.jpg|left|200px]]<br /><applet load="1e9i" size=" | + | [[Image:1e9i.jpg|left|200px]]<br /><applet load="1e9i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1e9i, resolution 2.48Å" /> | caption="1e9i, resolution 2.48Å" /> | ||
'''ENOLASE FROM E.COLI'''<br /> | '''ENOLASE FROM E.COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric | + | The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric unit of the C2 cell, and in one of the molecules, flexible loops close onto the active site. This closure mimics the conformation of the substrate-bound intermediate. A comparison of the structure of the E. coli enolase with the eukaryotic enolase structures available (lobster and yeast) indicates a high degree of conservation of the hydrophobic core and the subunit interface of this homodimeric enzyme. The dimer interface is enriched in charged residues compared with other protein homodimers, which may explain our observations from analytical ultracentrifugation that dimerisation is affected by ionic strength. The putative role of enolase in the RNA degradosome is discussed; although it was not possible to ascribe a specific role to it, a structural role is possible. |
==About this Structure== | ==About this Structure== | ||
| - | 1E9I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] Full crystallographic information is available from [http:// | + | 1E9I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E9I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Phosphopyruvate hydratase]] | [[Category: Phosphopyruvate hydratase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Carpousis, A | + | [[Category: Carpousis, A J.]] |
[[Category: Kuhnel, K.]] | [[Category: Kuhnel, K.]] | ||
[[Category: Luisi, B.]] | [[Category: Luisi, B.]] | ||
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[[Category: lyase]] | [[Category: lyase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:25:22 2008'' |
Revision as of 10:25, 21 February 2008
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ENOLASE FROM E.COLI
Overview
The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric unit of the C2 cell, and in one of the molecules, flexible loops close onto the active site. This closure mimics the conformation of the substrate-bound intermediate. A comparison of the structure of the E. coli enolase with the eukaryotic enolase structures available (lobster and yeast) indicates a high degree of conservation of the hydrophobic core and the subunit interface of this homodimeric enzyme. The dimer interface is enriched in charged residues compared with other protein homodimers, which may explain our observations from analytical ultracentrifugation that dimerisation is affected by ionic strength. The putative role of enolase in the RNA degradosome is discussed; although it was not possible to ascribe a specific role to it, a structural role is possible.
About this Structure
1E9I is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Phosphopyruvate hydratase, with EC number 4.2.1.11 Full crystallographic information is available from OCA.
Reference
Crystal structure of the Escherichia coli RNA degradosome component enolase., Kuhnel K, Luisi BF, J Mol Biol. 2001 Oct 26;313(3):583-92. PMID:11676541
Page seeded by OCA on Thu Feb 21 12:25:22 2008
