1eb6
From Proteopedia
| Line 4: | Line 4: | ||
==Overview== | ==Overview== | ||
| - | A data set from the metalloproteinase deuterolysin was collected at atomic | + | A data set from the metalloproteinase deuterolysin was collected at atomic resolution (1.0 A) with synchrotron radiation. The high resolution allowed the structure to be solved with the new direct-methods program ACORN using the coordinates of the Zn atom as a starting point. The phases obtained from ACORN were of sufficient quality to allow automated building to be carried out in ARP/wARP. Minimal manual rebuilding of the model was required and the structure determination was completed using the maximum-likelihood refinement program REFMAC. The whole process, starting from the processed and merged data and ending with a refined model, required less than 6 h of computational time. |
==About this Structure== | ==About this Structure== | ||
| Line 14: | Line 14: | ||
[[Category: Deuterolysin]] | [[Category: Deuterolysin]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Dodson, E | + | [[Category: Dodson, E J.]] |
[[Category: Jia-Xing, Y.]] | [[Category: Jia-Xing, Y.]] | ||
[[Category: Lehmbeck, J.]] | [[Category: Lehmbeck, J.]] | ||
| - | [[Category: Mcauley, K | + | [[Category: Mcauley, K E.]] |
| - | [[Category: Ostergaard, P | + | [[Category: Ostergaard, P R.]] |
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 27: | Line 27: | ||
[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:25:53 2008'' |
Revision as of 10:25, 21 February 2008
|
DEUTEROLYSIN FROM ASPERGILLUS ORYZAE
Overview
A data set from the metalloproteinase deuterolysin was collected at atomic resolution (1.0 A) with synchrotron radiation. The high resolution allowed the structure to be solved with the new direct-methods program ACORN using the coordinates of the Zn atom as a starting point. The phases obtained from ACORN were of sufficient quality to allow automated building to be carried out in ARP/wARP. Minimal manual rebuilding of the model was required and the structure determination was completed using the maximum-likelihood refinement program REFMAC. The whole process, starting from the processed and merged data and ending with a refined model, required less than 6 h of computational time.
About this Structure
1EB6 is a Single protein structure of sequence from Aspergillus oryzae with and as ligands. Active as Deuterolysin, with EC number 3.4.24.39 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
A quick solution: ab initio structure determination of a 19 kDa metalloproteinase using ACORN., McAuley KE, Jia-Xing Y, Dodson EJ, Lehmbeck J, Ostergaard PR, Wilson KS, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1571-8. Epub 2001, Oct 25. PMID:11679721
Page seeded by OCA on Thu Feb 21 12:25:53 2008
