1ebf
From Proteopedia
(New page: 200px<br /><applet load="1ebf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebf, resolution 2.30Å" /> '''HOMOSERINE DEHYDROGE...) |
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| - | [[Image:1ebf.jpg|left|200px]]<br /><applet load="1ebf" size=" | + | [[Image:1ebf.jpg|left|200px]]<br /><applet load="1ebf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ebf, resolution 2.30Å" /> | caption="1ebf, resolution 2.30Å" /> | ||
'''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+'''<br /> | '''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the antifungal drug target homoserine dehydrogenase (HSD) | + | The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry. |
==About this Structure== | ==About this Structure== | ||
| - | 1EBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NA and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http:// | + | 1EBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berghuis, A | + | [[Category: Berghuis, A M.]] |
[[Category: DeLaBarre, B.]] | [[Category: DeLaBarre, B.]] | ||
| - | [[Category: Thompson, P | + | [[Category: Thompson, P R.]] |
| - | [[Category: Wright, G | + | [[Category: Wright, G D.]] |
[[Category: NA]] | [[Category: NA]] | ||
[[Category: NAD]] | [[Category: NAD]] | ||
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[[Category: nad]] | [[Category: nad]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:06 2008'' |
Revision as of 10:26, 21 February 2008
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HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+
Overview
The structure of the antifungal drug target homoserine dehydrogenase (HSD) was determined from Saccharomyces cerevisiae in apo and holo forms, and as a ternary complex with bound products, by X-ray diffraction. The three forms show that the enzyme is a dimer, with each monomer composed of three regions, the nucleotide-binding region, the dimerization region and the catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the Rossmann fold. The novel folds impose a novel composition and arrangement of active site residues when compared to all other currently known oxidoreductases. This observation, in conjunction with site-directed mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
About this Structure
1EBF is a Single protein structure of sequence from Saccharomyces cerevisiae with and as ligands. Active as Homoserine dehydrogenase, with EC number 1.1.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:10700284
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