1ush
From Proteopedia
(New page: 200px<br /> <applet load="1ush" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ush, resolution 1.73Å" /> '''5'-NUCLEOTIDASE FRO...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1USH is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN, CO3 and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1USH OCA]]. | + | 1USH is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN, CO3 and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5]]. Structure known Active Site: ZNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1USH OCA]]. |
==Reference== | ==Reference== | ||
X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10331872 10331872] | X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10331872 10331872] | ||
| + | [[Category: 5'-nucleotidase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: udp-sugar hydrolase]] | [[Category: udp-sugar hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:56:07 2007'' |
Revision as of 11:51, 30 October 2007
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5'-NUCLEOTIDASE FROM E. COLI
Overview
The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known, as UDP-sugar hydrolase, has been determined at 1.7 A resolution. Two zinc, ions are present in the active site, which is located in a cleft between, two domains. The dimetal center and a catalytic Asp-His dyad are the main, players in the catalytic mechanism. Structure-based sequence comparisons, show that the structure also provides a model for animal 5'-NTs, which, together with other ectonucleotidases terminate the action of nucleotides, as extracellular signaling substances in the nervous system.
About this Structure
1USH is a [Single protein] structure of sequence from [Escherichia coli] with ZN, CO3 and SO4 as [ligands]. Active as [5'-nucleotidase], with EC number [3.1.3.5]. Structure known Active Site: ZNB. Full crystallographic information is available from [OCA].
Reference
X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site., Knofel T, Strater N, Nat Struct Biol. 1999 May;6(5):448-53. PMID:10331872
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