1egk

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(New page: 200px<br /><applet load="1egk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1egk, resolution 3.1&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1egk.gif|left|200px]]<br /><applet load="1egk" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1egk.gif|left|200px]]<br /><applet load="1egk" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1egk, resolution 3.1&Aring;" />
caption="1egk, resolution 3.1&Aring;" />
'''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION'''<br />
'''CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION'''<br />
==Overview==
==Overview==
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A crystal structure of a 108 nucleotide RNA-DNA complex containing a, four-way junction was solved at 3.1 A resolution. The structure of the, junction differs substantially from the "stacked-X" conformation observed, previously, due to a 135 degrees rotation of the branches. Comparison of, the two conformers provides insight into the factors contributing to the, flexibility of four-way junctions. The stacked-X conformation maximizes, base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes, electrostatic clashes at the expense of reduced base-stacking. Despite the, large rotation of the branches, both junction structures exhibit an, antiparallel arrangement of the continuous strands and opposite polarity, of the crossover strands.
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A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.
==About this Structure==
==About this Structure==
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1EGK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EGK OCA].
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1EGK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGK OCA].
==Reference==
==Reference==
Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10864501 10864501]
Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10864501 10864501]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Joyce, G.F.]]
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[[Category: Joyce, G F.]]
[[Category: Nowakowski, J.]]
[[Category: Nowakowski, J.]]
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[[Category: Shim, P.J.]]
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[[Category: Shim, P J.]]
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[[Category: Stout, C.D.]]
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[[Category: Stout, C D.]]
[[Category: MG]]
[[Category: MG]]
[[Category: 10-23 dna enzyme]]
[[Category: 10-23 dna enzyme]]
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[[Category: rna-dna complex]]
[[Category: rna-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:13:02 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:34 2008''

Revision as of 10:27, 21 February 2008

Image:1egk.gif

1egk, resolution 3.1Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF A NUCLEIC ACID FOUR-WAY JUNCTION

Overview

A crystal structure of a 108 nucleotide RNA-DNA complex containing a four-way junction was solved at 3.1 A resolution. The structure of the junction differs substantially from the "stacked-X" conformation observed previously, due to a 135 degrees rotation of the branches. Comparison of the two conformers provides insight into the factors contributing to the flexibility of four-way junctions. The stacked-X conformation maximizes base-stacking but causes unfavorable repulsion between phosphate groups, whereas the 135 degrees -rotated "crossed" conformation minimizes electrostatic clashes at the expense of reduced base-stacking. Despite the large rotation of the branches, both junction structures exhibit an antiparallel arrangement of the continuous strands and opposite polarity of the crossover strands.

About this Structure

1EGK is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Alternative conformations of a nucleic acid four-way junction., Nowakowski J, Shim PJ, Stout CD, Joyce GF, J Mol Biol. 2000 Jun 30;300(1):93-102. PMID:10864501

Page seeded by OCA on Thu Feb 21 12:27:34 2008

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