1eht
From Proteopedia
(New page: 200px<br /><applet load="1eht" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eht" /> '''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THE...) |
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| - | [[Image:1eht.jpg|left|200px]]<br /><applet load="1eht" size=" | + | [[Image:1eht.jpg|left|200px]]<br /><applet load="1eht" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1eht" /> | caption="1eht" /> | ||
'''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES'''<br /> | '''THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | To visualize the interplay of RNA structural interactions in a ligand | + | To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination. |
==About this Structure== | ==About this Structure== | ||
| - | 1EHT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with TEP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1EHT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=TEP:'>TEP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EHT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
| - | [[Category: Zimmermann, G | + | [[Category: Zimmermann, G R.]] |
[[Category: TEP]] | [[Category: TEP]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:55 2008'' |
Revision as of 10:27, 21 February 2008
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THEOPHYLLINE-BINDING RNA IN COMPLEX WITH THEOPHYLLINE, NMR, 10 STRUCTURES
Overview
To visualize the interplay of RNA structural interactions in a ligand binding site, we have determined the solution structure of a high affinity RNA-theophylline complex using NMR spectroscopy. The structure provides insight into the ability of this in vitro selected RNA to discriminate theophylline from the structurally similar molecule caffeine. Numerous RNA structural motifs combine to form a well-ordered binding pocket where an intricate network of hydrogen bonds and stacking interactions lock the theophylline into the complex. Two internal loops interact to form the binding site which consists of a sandwich of three base triples. The complex also contains novel base-zipper and 1-3-2 stacking motifs, in addition to an adenosine platform and a reversed sugar. An important feature of the RNA is that many of the conserved core residues participate in multiple overlapping tertiary interactions. This complex illustrates how interlocking structural motifs can be assembled into a highly specific ligand-binding site that possesses high levels of affinity and molecular discrimination.
About this Structure
1EHT is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Interlocking structural motifs mediate molecular discrimination by a theophylline-binding RNA., Zimmermann GR, Jenison RD, Wick CL, Simorre JP, Pardi A, Nat Struct Biol. 1997 Aug;4(8):644-9. PMID:9253414
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