1ej0
From Proteopedia
(New page: 200px<br /><applet load="1ej0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ej0, resolution 1.50Å" /> '''FTSJ RNA METHYLTRANS...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size=" | + | [[Image:1ej0.jpg|left|200px]]<br /><applet load="1ej0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ej0, resolution 1.50Å" /> | caption="1ej0, resolution 1.50Å" /> | ||
'''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE'''<br /> | '''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structural, biochemical, and genetic techniques were applied to | + | Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism. |
==About this Structure== | ==About this Structure== | ||
| - | 1EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG and SAM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1EJ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ0 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bardwell, J | + | [[Category: Bardwell, J C.A.]] |
[[Category: Bugl, H.]] | [[Category: Bugl, H.]] | ||
| - | [[Category: Fauman, E | + | [[Category: Fauman, E B.]] |
[[Category: Jakob, U.]] | [[Category: Jakob, U.]] | ||
| - | [[Category: Kushner, S | + | [[Category: Kushner, S R.]] |
| - | [[Category: Saper, M | + | [[Category: Saper, M A.]] |
| - | [[Category: Staker, B | + | [[Category: Staker, B L.]] |
[[Category: Zheng, F.]] | [[Category: Zheng, F.]] | ||
[[Category: HG]] | [[Category: HG]] | ||
| Line 30: | Line 30: | ||
[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:15 2008'' |
Revision as of 10:28, 21 February 2008
|
FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE
Overview
Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.
About this Structure
1EJ0 is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982
Page seeded by OCA on Thu Feb 21 12:28:15 2008
