1eiz
From Proteopedia
(New page: 200px<br /><applet load="1eiz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eiz, resolution 1.7Å" /> '''FTSJ RNA METHYLTRANSF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1eiz.jpg|left|200px]]<br /><applet load="1eiz" size=" | + | [[Image:1eiz.jpg|left|200px]]<br /><applet load="1eiz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1eiz, resolution 1.7Å" /> | caption="1eiz, resolution 1.7Å" /> | ||
'''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE'''<br /> | '''FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Structural, biochemical, and genetic techniques were applied to | + | Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism. |
==About this Structure== | ==About this Structure== | ||
| - | 1EIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1EIZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EIZ OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bardwell, J | + | [[Category: Bardwell, J C.A.]] |
[[Category: Bugl, H.]] | [[Category: Bugl, H.]] | ||
| - | [[Category: Fauman, E | + | [[Category: Fauman, E B.]] |
[[Category: Jakob, U.]] | [[Category: Jakob, U.]] | ||
| - | [[Category: Kushner, S | + | [[Category: Kushner, S R.]] |
| - | [[Category: Saper, M | + | [[Category: Saper, M A.]] |
| - | [[Category: Staker, B | + | [[Category: Staker, B L.]] |
[[Category: Zheng, F.]] | [[Category: Zheng, F.]] | ||
[[Category: SAM]] | [[Category: SAM]] | ||
| Line 29: | Line 29: | ||
[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:28:16 2008'' |
Revision as of 10:28, 21 February 2008
|
FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE
Overview
Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.
About this Structure
1EIZ is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
Reference
RNA methylation under heat shock control., Bugl H, Fauman EB, Staker BL, Zheng F, Kushner SR, Saper MA, Bardwell JC, Jakob U, Mol Cell. 2000 Aug;6(2):349-60. PMID:10983982
Page seeded by OCA on Thu Feb 21 12:28:16 2008
