1enn

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(New page: 200px<br /><applet load="1enn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1enn, resolution 0.89&Aring;" /> '''SOLVENT ORGANIZATION...)
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[[Image:1enn.gif|left|200px]]<br /><applet load="1enn" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1enn.gif|left|200px]]<br /><applet load="1enn" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1enn, resolution 0.89&Aring;" />
caption="1enn, resolution 0.89&Aring;" />
'''SOLVENT ORGANIZATION IN AN OLIGONUCLEOTIDE CRYSTAL: THE STRUCTURE OF D(GCGAATTCG)2 AT ATOMIC RESOLUTION'''<br />
'''SOLVENT ORGANIZATION IN AN OLIGONUCLEOTIDE CRYSTAL: THE STRUCTURE OF D(GCGAATTCG)2 AT ATOMIC RESOLUTION'''<br />
==Overview==
==Overview==
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We describe the crystal structure of d(GCGAATTCG) determined by x-ray, diffraction at atomic resolution level (0.89 A). The duplex structure is, practically identical to that described at 2.05 A resolution (Van, Meervelt, L., Vlieghe, D., Dautant, A., Gallois, B., Precigoux, G., and, Kennard, O. (1995) Nature 374, 742-744), however about half of the, phosphate groups show multiple conformations. The crystal has three, regions with different solvent structure. One of them contains several, ordered Mg(+2) ions and can be considered as an ionic crystal. A second, region is formed by a network of ordered water molecules with a polygonal, organization that binds three duplexes. The third region is formed by, channels of solvent in which very few ordered solvent molecules are, visible. The less ordered phosphates are found facing this channel. The, latter region provides a view of DNA with highly movable charges, both, negative phosphates and counterions, without a precise location.
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We describe the crystal structure of d(GCGAATTCG) determined by x-ray diffraction at atomic resolution level (0.89 A). The duplex structure is practically identical to that described at 2.05 A resolution (Van Meervelt, L., Vlieghe, D., Dautant, A., Gallois, B., Precigoux, G., and Kennard, O. (1995) Nature 374, 742-744), however about half of the phosphate groups show multiple conformations. The crystal has three regions with different solvent structure. One of them contains several ordered Mg(+2) ions and can be considered as an ionic crystal. A second region is formed by a network of ordered water molecules with a polygonal organization that binds three duplexes. The third region is formed by channels of solvent in which very few ordered solvent molecules are visible. The less ordered phosphates are found facing this channel. The latter region provides a view of DNA with highly movable charges, both negative phosphates and counterions, without a precise location.
==About this Structure==
==About this Structure==
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1ENN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG, CL and O as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 475D. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ENN OCA].
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1ENN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=O:'>O</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 475D. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ENN OCA].
==Reference==
==Reference==
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[[Category: Malinina, L.]]
[[Category: Malinina, L.]]
[[Category: Soler-Lopez, M.]]
[[Category: Soler-Lopez, M.]]
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[[Category: Subirana, J.A.]]
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[[Category: Subirana, J A.]]
[[Category: CL]]
[[Category: CL]]
[[Category: MG]]
[[Category: MG]]
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[[Category: ions]]
[[Category: ions]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:34:39 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:29:39 2008''

Revision as of 10:29, 21 February 2008

Image:1enn.gif

1enn, resolution 0.89Å

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SOLVENT ORGANIZATION IN AN OLIGONUCLEOTIDE CRYSTAL: THE STRUCTURE OF D(GCGAATTCG)2 AT ATOMIC RESOLUTION

Overview

We describe the crystal structure of d(GCGAATTCG) determined by x-ray diffraction at atomic resolution level (0.89 A). The duplex structure is practically identical to that described at 2.05 A resolution (Van Meervelt, L., Vlieghe, D., Dautant, A., Gallois, B., Precigoux, G., and Kennard, O. (1995) Nature 374, 742-744), however about half of the phosphate groups show multiple conformations. The crystal has three regions with different solvent structure. One of them contains several ordered Mg(+2) ions and can be considered as an ionic crystal. A second region is formed by a network of ordered water molecules with a polygonal organization that binds three duplexes. The third region is formed by channels of solvent in which very few ordered solvent molecules are visible. The less ordered phosphates are found facing this channel. The latter region provides a view of DNA with highly movable charges, both negative phosphates and counterions, without a precise location.

About this Structure

1ENN is a Protein complex structure of sequences from [1] with , and as ligands. This structure supersedes the now removed PDB entry 475D. Full crystallographic information is available from OCA.

Reference

Solvent organization in an oligonucleotide crystal. The structure of d(GCGAATTCG)2 at atomic resolution., Soler-Lopez M, Malinina L, Subirana JA, J Biol Chem. 2000 Jul 28;275(30):23034-44. PMID:10749874

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