1epy
From Proteopedia
(New page: 200px<br /><applet load="1epy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1epy, resolution 1.85Å" /> '''T4 LYSOZYME MUTANT, ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1epy.gif|left|200px]]<br /><applet load="1epy" size=" | + | [[Image:1epy.gif|left|200px]]<br /><applet load="1epy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1epy, resolution 1.85Å" /> | caption="1epy, resolution 1.85Å" /> | ||
'''T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H'''<br /> | '''T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H'''<br /> | ||
==Overview== | ==Overview== | ||
| - | It is not easy to find candidate sites within a given protein where the | + | It is not easy to find candidate sites within a given protein where the geometry of the polypeptide chain matches that of metal-binding sites in known protein structures. By choosing a location in T4 lysozyme that is inherently flexible, it was possible to engineer a two-histidine site that binds different divalent cations. Crystallographic analysis shows that the geometry of binding of zinc is distorted tetrahedral while that of cobalt and nickel is octahedral. Insofar as spectroscopic data can be measured, they indicate that similar modes of coordination are retained in solution. The two substitutions, Thr21 --> His and Thr142 --> His, lie, respectively, on the surface of the N- and C-terminal domains on opposite sides of the active site cleft. The design takes advantage of hinge-bending motion which allows the binding site to adapt to the most favorable ligand geometry for the metal. Introduction of the two histidines increases the melting temperature of the protein by 2.0 degrees C at pH 7.4. Metal binding further increases the melting temperature, but only by a small amount (up to 1.5 degrees C). A third substitution, Gln141 --> His, which could act as a third ligand in principle, does not do so, demonstrating the difficulty in mimicking naturally occurring metal-binding sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1EPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with SO4, CL and CO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1EPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EPY OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baase, W | + | [[Category: Baase, W A.]] |
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
| - | [[Category: Ostheimer, G | + | [[Category: Ostheimer, G J.]] |
| - | [[Category: Wray, J | + | [[Category: Wray, J W.]] |
| - | [[Category: Zhang, X | + | [[Category: Zhang, X J.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: CO]] | [[Category: CO]] | ||
| Line 24: | Line 24: | ||
[[Category: metal binding]] | [[Category: metal binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:20 2008'' |
Revision as of 10:30, 21 February 2008
|
T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H
Overview
It is not easy to find candidate sites within a given protein where the geometry of the polypeptide chain matches that of metal-binding sites in known protein structures. By choosing a location in T4 lysozyme that is inherently flexible, it was possible to engineer a two-histidine site that binds different divalent cations. Crystallographic analysis shows that the geometry of binding of zinc is distorted tetrahedral while that of cobalt and nickel is octahedral. Insofar as spectroscopic data can be measured, they indicate that similar modes of coordination are retained in solution. The two substitutions, Thr21 --> His and Thr142 --> His, lie, respectively, on the surface of the N- and C-terminal domains on opposite sides of the active site cleft. The design takes advantage of hinge-bending motion which allows the binding site to adapt to the most favorable ligand geometry for the metal. Introduction of the two histidines increases the melting temperature of the protein by 2.0 degrees C at pH 7.4. Metal binding further increases the melting temperature, but only by a small amount (up to 1.5 degrees C). A third substitution, Gln141 --> His, which could act as a third ligand in principle, does not do so, demonstrating the difficulty in mimicking naturally occurring metal-binding sites.
About this Structure
1EPY is a Single protein structure of sequence from Bacteriophage t4 with , and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Use of a non-rigid region in T4 lysozyme to design an adaptable metal-binding site., Wray JW, Baase WA, Ostheimer GJ, Zhang XJ, Matthews BW, Protein Eng. 2000 May;13(5):313-21. PMID:10835104
Page seeded by OCA on Thu Feb 21 12:30:20 2008
Categories: Bacteriophage t4 | Lysozyme | Single protein | Baase, W A. | Matthews, B W. | Ostheimer, G J. | Wray, J W. | Zhang, X J. | CL | CO | SO4 | Metal binding
