1eqp
From Proteopedia
(New page: 200px<br /><applet load="1eqp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eqp, resolution 1.9Å" /> '''EXO-B-(1,3)-GLUCANASE...) |
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| - | [[Image:1eqp.gif|left|200px]]<br /><applet load="1eqp" size=" | + | [[Image:1eqp.gif|left|200px]]<br /><applet load="1eqp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1eqp, resolution 1.9Å" /> | caption="1eqp, resolution 1.9Å" /> | ||
'''EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS'''<br /> | '''EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In some species of Candida the CUG codon is encoded as serine and not | + | In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production. |
==About this Structure== | ==About this Structure== | ||
| - | 1EQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Active as [http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] Full crystallographic information is available from [http:// | + | 1EQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Active as [http://en.wikipedia.org/wiki/Glucan_1,3-beta-glucosidase Glucan 1,3-beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.58 3.2.1.58] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Glucan 1,3-beta-glucosidase]] | [[Category: Glucan 1,3-beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Cutfield, J | + | [[Category: Cutfield, J F.]] |
| - | [[Category: Cutfield, S | + | [[Category: Cutfield, S M.]] |
| - | [[Category: Sullivan, P | + | [[Category: Sullivan, P A.]] |
[[Category: alternative codon usage]] | [[Category: alternative codon usage]] | ||
[[Category: candida albicans]] | [[Category: candida albicans]] | ||
[[Category: exoglucanase]] | [[Category: exoglucanase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:38 2008'' |
Revision as of 10:30, 21 February 2008
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EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS
Overview
In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.
About this Structure
1EQP is a Single protein structure of sequence from Candida albicans. Active as Glucan 1,3-beta-glucosidase, with EC number 3.2.1.58 Full crystallographic information is available from OCA.
Reference
Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase., Cutfield JF, Sullivan PA, Cutfield SM, Protein Eng. 2000 Oct;13(10):735-8. PMID:11112513
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