1esh

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(New page: 200px<br /><applet load="1esh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1esh" /> '''THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'...)
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[[Image:1esh.gif|left|200px]]<br /><applet load="1esh" size="350" color="white" frame="true" align="right" spinBox="true"
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'''THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA'''<br />
'''THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA'''<br />
==Overview==
==Overview==
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The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that, directs its RNA synthesis. A stem loop structure in this sequence, stem, loop C (SLC), was investigated using NMR, and correlated with its ability, to direct RNA synthesis by its replicase. SLC consists of two discrete, domains, a flexible stem with an internal loop and a rigid stem containing, a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only, one side of the flexible stem and a specific compact conformation of the, triloop. A high resolution structure of the triloop places the 5' adenine, out in solution, and the 3' adenine within the triloop, held tightly, through stacking and unusual hydrogen bonds. This high resolution, structure of an RNA promoter from a (+)-strand RNA virus provides new, insights into how the RNA-dependent RNA polymerase binds to the RNA to, initiate synthesis.
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The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis.
==About this Structure==
==About this Structure==
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1ESH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ESH OCA].
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1ESH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESH OCA].
==Reference==
==Reference==
RNA motifs that determine specificity between a viral replicase and its promoter., Kim CH, Kao CC, Tinoco I Jr, Nat Struct Biol. 2000 May;7(5):415-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10802741 10802741]
RNA motifs that determine specificity between a viral replicase and its promoter., Kim CH, Kao CC, Tinoco I Jr, Nat Struct Biol. 2000 May;7(5):415-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10802741 10802741]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Jr., I.Tinoco.]]
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[[Category: Jr., I Tinoco.]]
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[[Category: Kao, C.C.]]
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[[Category: Kao, C C.]]
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[[Category: Kim, C.H.]]
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[[Category: Kim, C H.]]
[[Category: brome mosaic virus]]
[[Category: brome mosaic virus]]
[[Category: rna]]
[[Category: rna]]
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[[Category: triloop]]
[[Category: triloop]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:45:19 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:04 2008''

Revision as of 10:31, 21 February 2008

Image:1esh.gif

1esh

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THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA

Overview

The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis.

About this Structure

1ESH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

RNA motifs that determine specificity between a viral replicase and its promoter., Kim CH, Kao CC, Tinoco I Jr, Nat Struct Biol. 2000 May;7(5):415-23. PMID:10802741

Page seeded by OCA on Thu Feb 21 12:31:04 2008

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