1ev7
From Proteopedia
(New page: 200px<br /><applet load="1ev7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ev7, resolution 2.38Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1ev7.gif|left|200px]]<br /><applet load="1ev7" size=" | + | [[Image:1ev7.gif|left|200px]]<br /><applet load="1ev7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ev7, resolution 2.38Å" /> | caption="1ev7, resolution 2.38Å" /> | ||
'''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''<br /> | '''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NAE:I is transformed from DNA endonuclease to DNA topoisomerase and | + | NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes. |
==About this Structure== | ==About this Structure== | ||
| - | 1EV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http:// | + | 1EV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Type II site-specific deoxyribonuclease]] | [[Category: Type II site-specific deoxyribonuclease]] | ||
[[Category: Chen, Y.]] | [[Category: Chen, Y.]] | ||
| - | [[Category: Colandene, J | + | [[Category: Colandene, J D.]] |
[[Category: Huai, Q.]] | [[Category: Huai, Q.]] | ||
[[Category: Luo, F.]] | [[Category: Luo, F.]] | ||
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[[Category: topoisomerase]] | [[Category: topoisomerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:49 2008'' |
Revision as of 10:31, 21 February 2008
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CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI
Overview
NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes.
About this Structure
1EV7 is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase., Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H, EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254
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