1ev6
From Proteopedia
(New page: 200px<br /> <applet load="1ev6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ev6, resolution 1.90Å" /> '''Structure of the mo...) |
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| - | [[Image:1ev6.gif|left|200px]]<br /> | + | [[Image:1ev6.gif|left|200px]]<br /><applet load="1ev6" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1ev6, resolution 1.90Å" /> | caption="1ev6, resolution 1.90Å" /> | ||
'''Structure of the monoclinic form of the M-cresol/insulin R6 hexamer'''<br /> | '''Structure of the monoclinic form of the M-cresol/insulin R6 hexamer'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structures of three R(6) human insulin hexamers have been determined. | + | The structures of three R(6) human insulin hexamers have been determined. Crystals of monoclinic m-cresol-insulin, monoclinic resorcinol-insulin and rhombohedral m-cresol-insulin diffracted to 1. 9, 1.9 and 1.78 A, respectively, and have been refined to residuals of 0.195, 0.179 and 0.200, respectively. In all three structures, a phenolic derivative is found to occupy the phenolic binding site, where it forms hydrogen bonds to the carbonyl O atom of CysA6 and the N atom of CysA11. Two additional phenolic derivative binding sites were identified within or between hexamers. The structures of all three hexamers are nearly identical, although a large displacement of the N-terminus of one B chain in both monoclinic structures results from coordination to a sodium ion which is located between symmetry-related hexamers. Other minor differences in structure arise from differences in packing in the monoclinic cell compared with the rhombohedral cell. Based upon the differences in conformation of the GluB13 side chains in T(6), T(3)R(f)(3) and R(6) hexamers, the deprotonation of these side chains appears to be associated with the T-->R conformational transition. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EV6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ZN, CL, NA and CRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1EV6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CRS:'>CRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV6 OCA]. |
==Reference== | ==Reference== | ||
R6 hexameric insulin complexed with m-cresol or resorcinol., Smith GD, Ciszak E, Magrum LA, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1541-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11092919 11092919] | R6 hexameric insulin complexed with m-cresol or resorcinol., Smith GD, Ciszak E, Magrum LA, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1541-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11092919 11092919] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Blessing, R | + | [[Category: Blessing, R H.]] |
[[Category: Ciszak, E.]] | [[Category: Ciszak, E.]] | ||
| - | [[Category: Magrum, L | + | [[Category: Magrum, L A.]] |
| - | [[Category: Pangborn, W | + | [[Category: Pangborn, W A.]] |
| - | [[Category: Smith, G | + | [[Category: Smith, G D.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: CRS]] | [[Category: CRS]] | ||
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[[Category: r6 hexameric insulin]] | [[Category: r6 hexameric insulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:48 2008'' |
Revision as of 10:31, 21 February 2008
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Structure of the monoclinic form of the M-cresol/insulin R6 hexamer
Contents |
Overview
The structures of three R(6) human insulin hexamers have been determined. Crystals of monoclinic m-cresol-insulin, monoclinic resorcinol-insulin and rhombohedral m-cresol-insulin diffracted to 1. 9, 1.9 and 1.78 A, respectively, and have been refined to residuals of 0.195, 0.179 and 0.200, respectively. In all three structures, a phenolic derivative is found to occupy the phenolic binding site, where it forms hydrogen bonds to the carbonyl O atom of CysA6 and the N atom of CysA11. Two additional phenolic derivative binding sites were identified within or between hexamers. The structures of all three hexamers are nearly identical, although a large displacement of the N-terminus of one B chain in both monoclinic structures results from coordination to a sodium ion which is located between symmetry-related hexamers. Other minor differences in structure arise from differences in packing in the monoclinic cell compared with the rhombohedral cell. Based upon the differences in conformation of the GluB13 side chains in T(6), T(3)R(f)(3) and R(6) hexamers, the deprotonation of these side chains appears to be associated with the T-->R conformational transition.
Disease
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]
About this Structure
1EV6 is a Protein complex structure of sequences from [1] with , , and as ligands. Full crystallographic information is available from OCA.
Reference
R6 hexameric insulin complexed with m-cresol or resorcinol., Smith GD, Ciszak E, Magrum LA, Pangborn WA, Blessing RH, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1541-8. PMID:11092919
Page seeded by OCA on Thu Feb 21 12:31:48 2008
Categories: Protein complex | Blessing, R H. | Ciszak, E. | Magrum, L A. | Pangborn, W A. | Smith, G D. | CL | CRS | NA | ZN | R6 hexameric insulin
