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1ewy
From Proteopedia
(New page: 200px<br /><applet load="1ewy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ewy, resolution 2.38Å" /> '''ANABAENA PCC7119 FER...) |
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| - | [[Image:1ewy.jpg|left|200px]]<br /><applet load="1ewy" size=" | + | [[Image:1ewy.jpg|left|200px]]<br /><applet load="1ewy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ewy, resolution 2.38Å" /> | caption="1ewy, resolution 2.38Å" /> | ||
'''ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX'''<br /> | '''ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor | + | Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A and the space group is P2(1)2(1)2(1). The crystal structure has been solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the crystal was treated as a merohedral twin. The asymmetric unit contains two FNR molecules and one ferredoxin molecule. The packing of the FNR molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides the first crystallographic model of a dissociable complex between FNR and Fd. |
==About this Structure== | ==About this Structure== | ||
| - | 1EWY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nostoc_sp._pcc_7119 Nostoc sp. pcc 7119] with FAD and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http:// | + | 1EWY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nostoc_sp._pcc_7119 Nostoc sp. pcc 7119] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nostoc sp. pcc 7119]] | [[Category: Nostoc sp. pcc 7119]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Charon, M | + | [[Category: Charon, M H.]] |
[[Category: Frey, M.]] | [[Category: Frey, M.]] | ||
[[Category: Morales, R.]] | [[Category: Morales, R.]] | ||
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[[Category: reductase]] | [[Category: reductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:24 2008'' |
Revision as of 10:32, 21 February 2008
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ANABAENA PCC7119 FERREDOXIN:FERREDOXIN-NADP+-REDUCTASE COMPLEX
Overview
Ferredoxin-NADP(+) reductase (FNR) and its physiological electron donor ferredoxin (Fd) from the cyanobacterium Anabaena PCC7119 have been co-crystallized. The unit-cell parameters are a = b = 63.72, c = 158.02 A and the space group is P2(1)2(1)2(1). The crystal structure has been solved with 2.4 A resolution synchrotron data by molecular replacement, anomalous dispersion and R(min) search methods. For the computations, the crystal was treated as a merohedral twin. The asymmetric unit contains two FNR molecules and one ferredoxin molecule. The packing of the FNR molecules displays a nearly tetragonal symmetry (space group P4(3)2(1)2), whereas the ferredoxin arrangement is orthorhombic. This study provides the first crystallographic model of a dissociable complex between FNR and Fd.
About this Structure
1EWY is a Protein complex structure of sequences from Nostoc sp. pcc 7119 with and as ligands. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the interaction between the ferredoxin-NADP+ reductase and ferredoxin from the cyanobacterium Anabaena: looking for the elusive ferredoxin molecule., Morales R, Kachalova G, Vellieux F, Charon MH, Frey M, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1408-12. PMID:11053838
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