1eyx

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(New page: 200px<br /><applet load="1eyx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyx, resolution 2.25&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1eyx.gif|left|200px]]<br /><applet load="1eyx" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1eyx, resolution 2.25&Aring;" />
caption="1eyx, resolution 2.25&Aring;" />
'''CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS'''<br />
'''CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS'''<br />
==Overview==
==Overview==
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R-phycoerythrin, a light-harvesting component from the red algae, Gracilaria chilensis, was crystallized by vapour diffusion using ammonium, sulfate as precipitant agent. Red crystals grew after one week at 293 K, and diffracted to 2.70 A resolution. Three serial macroseeding assays were, necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x, 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron, radiation at beamline BM14 of the European Synchrotron Radiation Facility, (ESRF) at Grenoble, France and was used for structure determination. Data, were collected at 100 K to a completeness of 98.6%. The crystal was, trigonal, space group R3, with unit-cell parameters a = b = 187.3, c =, 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the, CCP4 suite of programs indicated that the crystal was twinned, ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe, using the R-phycoerythrin from Polysiphonia urceolata [Chang et al., (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to, overcome the twinning problem, SHELX97 was used for the crystallographic, refinement. The twin fraction was 0.48, indicating a nearly perfect, hemihedrally twinned crystal. The final R(work) and R(free) factors are, 0.16 and 0.25, respectively. All the residues and chromophores of the, alpha- and beta-chains are well defined in the electron-density maps. Some, residues belonging to the gamma-linker are also recognizable.
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R-phycoerythrin, a light-harvesting component from the red algae Gracilaria chilensis, was crystallized by vapour diffusion using ammonium sulfate as precipitant agent. Red crystals grew after one week at 293 K and diffracted to 2.70 A resolution. Three serial macroseeding assays were necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron radiation at beamline BM14 of the European Synchrotron Radiation Facility (ESRF) at Grenoble, France and was used for structure determination. Data were collected at 100 K to a completeness of 98.6%. The crystal was trigonal, space group R3, with unit-cell parameters a = b = 187.3, c = 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the CCP4 suite of programs indicated that the crystal was twinned ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe using the R-phycoerythrin from Polysiphonia urceolata [Chang et al. (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to overcome the twinning problem, SHELX97 was used for the crystallographic refinement. The twin fraction was 0.48, indicating a nearly perfect hemihedrally twinned crystal. The final R(work) and R(free) factors are 0.16 and 0.25, respectively. All the residues and chromophores of the alpha- and beta-chains are well defined in the electron-density maps. Some residues belonging to the gamma-linker are also recognizable.
==About this Structure==
==About this Structure==
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1EYX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gracilaria_chilensis Gracilaria chilensis] with SO4, BLA, CYC and PUB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA].
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1EYX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gracilaria_chilensis Gracilaria chilensis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BLA:'>BLA</scene>, <scene name='pdbligand=CYC:'>CYC</scene> and <scene name='pdbligand=PUB:'>PUB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA].
==Reference==
==Reference==
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[[Category: Bunster, M.]]
[[Category: Bunster, M.]]
[[Category: Contreras-Martel, C.]]
[[Category: Contreras-Martel, C.]]
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[[Category: Fontecilla-Camps, J.C.]]
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[[Category: Fontecilla-Camps, J C.]]
[[Category: Legrand, P.]]
[[Category: Legrand, P.]]
[[Category: Martinez-Oyanedel, J.]]
[[Category: Martinez-Oyanedel, J.]]
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[[Category: twin]]
[[Category: twin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:59 2008''

Revision as of 10:32, 21 February 2008

Image:1eyx.gif

1eyx, resolution 2.25Å

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CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS

Overview

R-phycoerythrin, a light-harvesting component from the red algae Gracilaria chilensis, was crystallized by vapour diffusion using ammonium sulfate as precipitant agent. Red crystals grew after one week at 293 K and diffracted to 2.70 A resolution. Three serial macroseeding assays were necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron radiation at beamline BM14 of the European Synchrotron Radiation Facility (ESRF) at Grenoble, France and was used for structure determination. Data were collected at 100 K to a completeness of 98.6%. The crystal was trigonal, space group R3, with unit-cell parameters a = b = 187.3, c = 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the CCP4 suite of programs indicated that the crystal was twinned ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe using the R-phycoerythrin from Polysiphonia urceolata [Chang et al. (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to overcome the twinning problem, SHELX97 was used for the crystallographic refinement. The twin fraction was 0.48, indicating a nearly perfect hemihedrally twinned crystal. The final R(work) and R(free) factors are 0.16 and 0.25, respectively. All the residues and chromophores of the alpha- and beta-chains are well defined in the electron-density maps. Some residues belonging to the gamma-linker are also recognizable.

About this Structure

1EYX is a Protein complex structure of sequences from Gracilaria chilensis with , , and as ligands. Full crystallographic information is available from OCA.

Reference

Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning., Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:11134927

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