1ezn
From Proteopedia
(New page: 200px<br /><applet load="1ezn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ezn" /> '''SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTI...) |
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| - | [[Image:1ezn.gif|left|200px]]<br /><applet load="1ezn" size=" | + | [[Image:1ezn.gif|left|200px]]<br /><applet load="1ezn" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION'''<br /> | '''SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of a DNA three-way junction (3H) containing two | + | The solution structure of a DNA three-way junction (3H) containing two unpaired thymidine bases at the branch site (3HT2), was determined by NMR. Arms A and B of the 3HT2 form a quasi-continuous stacked helix, which is underwound at the junction and has an increased helical rise. The unstacked arm C forms an acute angle of approximately 55 degrees with the unique arm A. The stacking of the unpaired thymidine bases on arm C resembles the folding of hairpin loops. From this data, combined with the reported stacking behavior of 23 other 3HS2 s, two rules are derived that together correctly reproduce their stacking preference. These rules predict, from the sequence of any 3HS2, its stacking preference. The structure also suggests a plausible mechanism for structure-specific recognition of branched nucleic acids by proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1EZN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1EZN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Altona, C.]] | [[Category: Altona, C.]] | ||
| - | [[Category: Buuren, B | + | [[Category: Buuren, B N.M van.]] |
| - | [[Category: Ippel, J | + | [[Category: Ippel, J H.]] |
| - | [[Category: Overmars, F | + | [[Category: Overmars, F J.]] |
| - | [[Category: Wijmenga, S | + | [[Category: Wijmenga, S S.]] |
[[Category: dna]] | [[Category: dna]] | ||
[[Category: four-way junction]] | [[Category: four-way junction]] | ||
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[[Category: three-way junction]] | [[Category: three-way junction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:13 2008'' |
Revision as of 10:33, 21 February 2008
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SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION
Overview
The solution structure of a DNA three-way junction (3H) containing two unpaired thymidine bases at the branch site (3HT2), was determined by NMR. Arms A and B of the 3HT2 form a quasi-continuous stacked helix, which is underwound at the junction and has an increased helical rise. The unstacked arm C forms an acute angle of approximately 55 degrees with the unique arm A. The stacking of the unpaired thymidine bases on arm C resembles the folding of hairpin loops. From this data, combined with the reported stacking behavior of 23 other 3HS2 s, two rules are derived that together correctly reproduce their stacking preference. These rules predict, from the sequence of any 3HS2, its stacking preference. The structure also suggests a plausible mechanism for structure-specific recognition of branched nucleic acids by proteins.
About this Structure
1EZN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection., van Buuren BN, Overmars FJ, Ippel JH, Altona C, Wijmenga SS, J Mol Biol. 2000 Dec 1;304(3):371-83. PMID:11090280
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