1f0v
From Proteopedia
(New page: 200px<br /><applet load="1f0v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f0v, resolution 1.7Å" /> '''Crystal structure of ...) |
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| - | [[Image:1f0v.gif|left|200px]]<br /><applet load="1f0v" size=" | + | [[Image:1f0v.gif|left|200px]]<br /><applet load="1f0v" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f0v, resolution 1.7Å" /> | caption="1f0v, resolution 1.7Å" /> | ||
'''Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping'''<br /> | '''Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bovine pancreatic ribonuclease (RNase A) forms two types of dimers (a | + | Bovine pancreatic ribonuclease (RNase A) forms two types of dimers (a major and a minor component) upon concentration in mild acid. These two dimers exhibit different biophysical and biochemical properties. Earlier we reported that the minor dimer forms by swapping its N-terminal alpha-helix with that of an identical molecule. Here we find that the major dimer forms by swapping its C-terminal beta-strand, thus revealing the first example of three-dimensional (3D) domain swapping taking place in different parts of the same protein. This feature permits RNase A to form tightly bonded higher oligomers. The hinge loop of the major dimer, connecting the swapped beta-strand to the protein core, resembles a short segment of the polar zipper proposed by Perutz and suggests a model for aggregate formation by 3D domain swapping with a polar zipper. |
==About this Structure== | ==About this Structure== | ||
| - | 1F0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with PO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http:// | + | 1F0V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0V OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Eisenberg, D | + | [[Category: Eisenberg, D S.]] |
[[Category: Gotte, G.]] | [[Category: Gotte, G.]] | ||
[[Category: Libonati, M.]] | [[Category: Libonati, M.]] | ||
| - | [[Category: Liu, Y | + | [[Category: Liu, Y S.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
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[[Category: x-ray]] | [[Category: x-ray]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:37 2008'' |
Revision as of 10:33, 21 February 2008
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Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping
Overview
Bovine pancreatic ribonuclease (RNase A) forms two types of dimers (a major and a minor component) upon concentration in mild acid. These two dimers exhibit different biophysical and biochemical properties. Earlier we reported that the minor dimer forms by swapping its N-terminal alpha-helix with that of an identical molecule. Here we find that the major dimer forms by swapping its C-terminal beta-strand, thus revealing the first example of three-dimensional (3D) domain swapping taking place in different parts of the same protein. This feature permits RNase A to form tightly bonded higher oligomers. The hinge loop of the major dimer, connecting the swapped beta-strand to the protein core, resembles a short segment of the polar zipper proposed by Perutz and suggests a model for aggregate formation by 3D domain swapping with a polar zipper.
About this Structure
1F0V is a Single protein structure of sequence from Bos taurus with and as ligands. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
Reference
A domain-swapped RNase A dimer with implications for amyloid formation., Liu Y, Gotte G, Libonati M, Eisenberg D, Nat Struct Biol. 2001 Mar;8(3):211-4. PMID:11224563
Page seeded by OCA on Thu Feb 21 12:33:37 2008
Categories: Bos taurus | Pancreatic ribonuclease | Single protein | Eisenberg, D S. | Gotte, G. | Libonati, M. | Liu, Y S. | GOL | PO4 | Bovine pancreas | Crystal | Domain swapping | Ribonuclease | Structure | X-ray
