1f2l
From Proteopedia
(New page: 200px<br /> <applet load="1f2l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f2l, resolution 2.00Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1f2l.gif|left|200px]]<br /> | + | [[Image:1f2l.gif|left|200px]]<br /><applet load="1f2l" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1f2l" size=" | + | |
caption="1f2l, resolution 2.00Å" /> | caption="1f2l, resolution 2.00Å" /> | ||
'''CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE'''<br /> | '''CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Fractalkine, or neurotactin, is a chemokine that is present in endothelial | + | Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood. |
==About this Structure== | ==About this Structure== | ||
| - | 1F2L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1F2L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2L OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Handel, T | + | [[Category: Handel, T M.]] |
| - | [[Category: Hoover, D | + | [[Category: Hoover, D M.]] |
[[Category: Lubkowski, J.]] | [[Category: Lubkowski, J.]] | ||
| - | [[Category: Mizoue, L | + | [[Category: Mizoue, L S.]] |
[[Category: chemoattractant]] | [[Category: chemoattractant]] | ||
[[Category: fractalkine]] | [[Category: fractalkine]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:11 2008'' |
Revision as of 10:34, 21 February 2008
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CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE
Overview
Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood.
About this Structure
1F2L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement., Hoover DM, Mizoue LS, Handel TM, Lubkowski J, J Biol Chem. 2000 Jul 28;275(30):23187-93. PMID:10770945
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