This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1f3s
From Proteopedia
(New page: 200px<br /><applet load="1f3s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f3s" /> '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size=" | + | [[Image:1f3s.gif|left|200px]]<br /><applet load="1f3s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f3s" /> | caption="1f3s" /> | ||
'''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br /> | '''SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to | + | We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit. |
==About this Structure== | ==About this Structure== | ||
| - | 1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1F3S is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3S OCA]. |
==Reference== | ==Reference== | ||
| Line 16: | Line 16: | ||
[[Category: Kettani, A.]] | [[Category: Kettani, A.]] | ||
[[Category: Majumdar, A.]] | [[Category: Majumdar, A.]] | ||
| - | [[Category: Patel, D | + | [[Category: Patel, D J.]] |
[[Category: Skripkin, E.]] | [[Category: Skripkin, E.]] | ||
[[Category: 15n-labeled dna]] | [[Category: 15n-labeled dna]] | ||
| Line 24: | Line 24: | ||
[[Category: uniform 13c]] | [[Category: uniform 13c]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:29 2008'' |
Revision as of 10:34, 21 February 2008
|
SOLUTION STRUCTURE OF DNA SEQUENCE GGGTTCAGG FORMS GGGG TETRADE AND G(C-A) TRIAD.
Overview
We have designed a DNA sequence, d(G-G-G-T-T-C-A-G-G), which dimerizes to form a 2-fold symmetric G-quadruplex in which G(syn). G(anti).G(syn).G(anti) tetrads are sandwiched between all trans G. (C-A) triads. The NMR-based solution structural analysis was greatly aided by monitoring hydrogen bond alignments across N-H...N and N-H...O==C hydrogen bonds within the triad and tetrad, in a uniformly ((13)C,(15)N)-labeled sample of the d(G-G-G-T-T-C-A-G-G) sequence. The solution structure establishes that the guanine base-pairs with the cytosine through Watson-Crick G.C pair formation and with adenine through sheared G.A mismatch formation within the G.(C-A) triad. A model of triad DNA was constructed that contains the experimentally determined G.(C-A) triad alignment as the repeating stacked unit.
About this Structure
1F3S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A two-stranded template-based approach to G.(C-A) triad formation: designing novel structural elements into an existing DNA framework., Kettani A, Basu G, Gorin A, Majumdar A, Skripkin E, Patel DJ, J Mol Biol. 2000 Aug 4;301(1):129-46. PMID:10926497
Page seeded by OCA on Thu Feb 21 12:34:29 2008
