2cbf
From Proteopedia
(New page: 200px<br /> <applet load="2cbf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cbf, resolution 3.1Å" /> '''THE X-RAY STRUCTURE ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]] with SAH as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]]. | + | 2CBF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]] with SAH as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133]]. Structure known Active Sites: FLP and SAH. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]]. |
==Reference== | ==Reference== | ||
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665173 9665173] | The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9665173 9665173] | ||
[[Category: Bacillus megaterium]] | [[Category: Bacillus megaterium]] | ||
| + | [[Category: Precorrin-4 C(11)-methyltransferase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Raux, E.]] | [[Category: Raux, E.]] | ||
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[[Category: precorrin-4 methyltransferase]] | [[Category: precorrin-4 methyltransferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:00:33 2007'' |
Revision as of 11:55, 30 October 2007
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THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF
Overview
Biosynthesis of the corrin ring of vitamin B12 requires the action of six, S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely, related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been, determined to a resolution of 2.4 A. CbiF contains two alphabeta domains, forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The, location of AdoHcy and a number of conserved residues, helps define the, precorrin binding site. A second crystal form determined at 3.1 A, resolution highlights the flexibility of two loops around this site. CbiF, employs a unique mode of AdoHcy binding and represents a new class of, transmethylase.
About this Structure
2CBF is a [Single protein] structure of sequence from [Bacillus megaterium] with SAH as [ligand]. Active as [Precorrin-4 C(11)-methyltransferase], with EC number [2.1.1.133]. Structure known Active Sites: FLP and SAH. Full crystallographic information is available from [OCA].
Reference
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173
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