1f5u
From Proteopedia
(New page: 200px<br /><applet load="1f5u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5u" /> '''SOLUTION STRUCTURE OF THE KISSING DIMER OF H...) |
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| - | [[Image:1f5u.gif|left|200px]]<br /><applet load="1f5u" size=" | + | [[Image:1f5u.gif|left|200px]]<br /><applet load="1f5u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1f5u" /> | caption="1f5u" /> | ||
'''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA'''<br /> | '''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The dimerization of viral RNA through noncovalent interactions at their 5' | + | The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs. |
==About this Structure== | ==About this Structure== | ||
| - | 1F5U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1F5U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. |
==Reference== | ==Reference== | ||
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10931958 10931958] | A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10931958 10931958] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Jr., I | + | [[Category: Jr., I Tinoco.]] |
| - | [[Category: Kim, C | + | [[Category: Kim, C H.]] |
[[Category: dimerization]] | [[Category: dimerization]] | ||
[[Category: kissing dimer]] | [[Category: kissing dimer]] | ||
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[[Category: tetraloop]] | [[Category: tetraloop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:11 2008'' |
Revision as of 10:35, 21 February 2008
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SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA
Overview
The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
About this Structure
1F5U is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958
Page seeded by OCA on Thu Feb 21 12:35:11 2008
