1f7y

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(New page: 200px<br /><applet load="1f7y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f7y, resolution 2.80&Aring;" /> '''THE CRYSTAL STRUCTUR...)
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[[Image:1f7y.gif|left|200px]]<br /><applet load="1f7y" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1f7y, resolution 2.80&Aring;" />
caption="1f7y, resolution 2.80&Aring;" />
'''THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY'''<br />
'''THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY'''<br />
==Overview==
==Overview==
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All large structured RNAs contain hairpin motifs made of a stem closed by, several looped nucleotides. The most frequent loop motif is the UUCG one., This motif belongs to the tetraloop family and has the peculiarity of, being highly thermodynamically stable. Here, we report the first crystal, structure of two UUCG tetraloops embedded in a larger RNA-protein complex, solved at 2.8 A resolution. The two loops present in the asymmetric unit, are in a different crystal packing environment but, nevertheless, have an, identical conformation. The observed structure is globally close to that, obtained in solution by nuclear magnetic resonance. However, subtle, differences point to a more detailed picture of the role played by, 2'-hydroxyl groups in stabilising this tetraloop.
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All large structured RNAs contain hairpin motifs made of a stem closed by several looped nucleotides. The most frequent loop motif is the UUCG one. This motif belongs to the tetraloop family and has the peculiarity of being highly thermodynamically stable. Here, we report the first crystal structure of two UUCG tetraloops embedded in a larger RNA-protein complex solved at 2.8 A resolution. The two loops present in the asymmetric unit are in a different crystal packing environment but, nevertheless, have an identical conformation. The observed structure is globally close to that obtained in solution by nuclear magnetic resonance. However, subtle differences point to a more detailed picture of the role played by 2'-hydroxyl groups in stabilising this tetraloop.
==About this Structure==
==About this Structure==
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1F7Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG, NA and K as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F7Y OCA].
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1F7Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7Y OCA].
==Reference==
==Reference==
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[[Category: x-ray]]
[[Category: x-ray]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:41:34 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:35:51 2008''

Revision as of 10:35, 21 February 2008

Image:1f7y.gif

1f7y, resolution 2.80Å

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THE CRYSTAL STRUCTURE OF TWO UUCG LOOPS HIGHLIGHTS THE ROLE PLAYED BY 2'-HYDROXYL GROUPS IN ITS UNUSUAL STABILITY

Overview

All large structured RNAs contain hairpin motifs made of a stem closed by several looped nucleotides. The most frequent loop motif is the UUCG one. This motif belongs to the tetraloop family and has the peculiarity of being highly thermodynamically stable. Here, we report the first crystal structure of two UUCG tetraloops embedded in a larger RNA-protein complex solved at 2.8 A resolution. The two loops present in the asymmetric unit are in a different crystal packing environment but, nevertheless, have an identical conformation. The observed structure is globally close to that obtained in solution by nuclear magnetic resonance. However, subtle differences point to a more detailed picture of the role played by 2'-hydroxyl groups in stabilising this tetraloop.

About this Structure

1F7Y is a Single protein structure of sequence from Thermus thermophilus with , and as ligands. Full crystallographic information is available from OCA.

Reference

The crystal structure of UUCG tetraloop., Ennifar E, Nikulin A, Tishchenko S, Serganov A, Nevskaya N, Garber M, Ehresmann B, Ehresmann C, Nikonov S, Dumas P, J Mol Biol. 2000 Nov 17;304(1):35-42. PMID:11071808

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