1fcw
From Proteopedia
(New page: 200px<br /><applet load="1fcw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fcw, resolution 17.0Å" /> '''TRNA POSITIONS DURIN...) |
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| - | [[Image:1fcw.gif|left|200px]]<br /><applet load="1fcw" size=" | + | [[Image:1fcw.gif|left|200px]]<br /><applet load="1fcw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fcw, resolution 17.0Å" /> | caption="1fcw, resolution 17.0Å" /> | ||
'''TRNA POSITIONS DURING THE ELONGATION CYCLE'''<br /> | '''TRNA POSITIONS DURING THE ELONGATION CYCLE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Three-dimensional cryomaps have been reconstructed for tRNA-ribosome | + | Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible. |
==About this Structure== | ==About this Structure== | ||
| - | 1FCW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1FCW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Agrawal, R | + | [[Category: Agrawal, R K.]] |
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
| - | [[Category: Grassucci, R | + | [[Category: Grassucci, R A.]] |
| - | [[Category: Nierhaus, K | + | [[Category: Nierhaus, K H.]] |
[[Category: Penczek, P.]] | [[Category: Penczek, P.]] | ||
| - | [[Category: Spahn, C | + | [[Category: Spahn, C M.T.]] |
[[Category: cryo-electron microscopy]] | [[Category: cryo-electron microscopy]] | ||
[[Category: elongation cycle]] | [[Category: elongation cycle]] | ||
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[[Category: trna binding sites]] | [[Category: trna binding sites]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:24 2008'' |
Revision as of 10:37, 21 February 2008
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TRNA POSITIONS DURING THE ELONGATION CYCLE
Overview
Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
About this Structure
1FCW is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:10931859
Page seeded by OCA on Thu Feb 21 12:37:24 2008
