1feu
From Proteopedia
(New page: 200px<br /><applet load="1feu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1feu, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1feu.gif|left|200px]]<br /><applet load="1feu" size=" | + | [[Image:1feu.gif|left|200px]]<br /><applet load="1feu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1feu, resolution 2.3Å" /> | caption="1feu, resolution 2.3Å" /> | ||
'''CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.'''<br /> | '''CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Thermus thermophilus ribosomal protein TL5 in | + | The crystal structure of Thermus thermophilus ribosomal protein TL5 in complex with a fragment of Escherichia coli 5S rRNA has been determined at 2.3 A resolution. The protein consists of two domains. The structure of the N-terminal domain is close to the structure of E. coli ribosomal protein L25, but the C-terminal domain represents a new fold composed of seven beta-strands connected by long loops. TL5 binds to the RNA through its N-terminal domain, whereas the C-terminal domain is not included in this interaction. Cd(2+) ions, the presence of which improved the crystal quality significantly, bind only to the protein component of the complex and stabilize the protein molecule itself and the interactions between the two molecules in the asymmetric unit of the crystal. The TL5 sequence reveals homology to the so-called general stress protein CTC. The hydrophobic cores which stabilize both TL5 domains are highly conserved in CTC proteins. Thus, all CTC proteins may fold with a topology close to that of TL5. |
==About this Structure== | ==About this Structure== | ||
| - | 1FEU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with CD and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1FEU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Al-Karadaghi, S.]] | [[Category: Al-Karadaghi, S.]] | ||
| - | [[Category: Fedorov, R | + | [[Category: Fedorov, R V.]] |
| - | [[Category: Fomenkova, N | + | [[Category: Fomenkova, N P.]] |
| - | [[Category: Garber, M | + | [[Category: Garber, M B.]] |
| - | [[Category: Gongadze, G | + | [[Category: Gongadze, G M.]] |
[[Category: Kristensen, O.]] | [[Category: Kristensen, O.]] | ||
[[Category: Laurberg, M.]] | [[Category: Laurberg, M.]] | ||
[[Category: Liljas, A.]] | [[Category: Liljas, A.]] | ||
| - | [[Category: Meshcheryakov, V | + | [[Category: Meshcheryakov, V A.]] |
| - | [[Category: Nevskaya, N | + | [[Category: Nevskaya, N A.]] |
| - | [[Category: Nikonov, S | + | [[Category: Nikonov, S V.]] |
[[Category: Selmer, M.]] | [[Category: Selmer, M.]] | ||
[[Category: CD]] | [[Category: CD]] | ||
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[[Category: general stress protein ctc]] | [[Category: general stress protein ctc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:37:52 2008'' |
Revision as of 10:37, 21 February 2008
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CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.
Overview
The crystal structure of Thermus thermophilus ribosomal protein TL5 in complex with a fragment of Escherichia coli 5S rRNA has been determined at 2.3 A resolution. The protein consists of two domains. The structure of the N-terminal domain is close to the structure of E. coli ribosomal protein L25, but the C-terminal domain represents a new fold composed of seven beta-strands connected by long loops. TL5 binds to the RNA through its N-terminal domain, whereas the C-terminal domain is not included in this interaction. Cd(2+) ions, the presence of which improved the crystal quality significantly, bind only to the protein component of the complex and stabilize the protein molecule itself and the interactions between the two molecules in the asymmetric unit of the crystal. The TL5 sequence reveals homology to the so-called general stress protein CTC. The hydrophobic cores which stabilize both TL5 domains are highly conserved in CTC proteins. Thus, all CTC proteins may fold with a topology close to that of TL5.
About this Structure
1FEU is a Single protein structure of sequence from Thermus thermophilus with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins., Fedorov R, Meshcheryakov V, Gongadze G, Fomenkova N, Nevskaya N, Selmer M, Laurberg M, Kristensen O, Al-Karadaghi S, Liljas A, Garber M, Nikonov S, Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):968-76. Epub 2001, Jun 21. PMID:11418764
Page seeded by OCA on Thu Feb 21 12:37:52 2008
Categories: Single protein | Thermus thermophilus | Al-Karadaghi, S. | Fedorov, R V. | Fomenkova, N P. | Garber, M B. | Gongadze, G M. | Kristensen, O. | Laurberg, M. | Liljas, A. | Meshcheryakov, V A. | Nevskaya, N A. | Nikonov, S V. | Selmer, M. | CD | MG | 5s rrna-protein complex | Cadmium ions | General stress protein ctc
